DnaA将DNA复制与两个细胞周期主调控因子的表达联系起来。
DnaA couples DNA replication and the expression of two cell cycle master regulators.
作者信息
Collier Justine, Murray Sean Richard, Shapiro Lucy
机构信息
Department of Developmental Biology, School of Medicine, Stanford University Medical Center, Beckman Center, Stanford, CA 94305, USA.
出版信息
EMBO J. 2006 Jan 25;25(2):346-56. doi: 10.1038/sj.emboj.7600927. Epub 2006 Jan 5.
Abstract
Cell cycle progression in Caulobacter is driven by the master transcriptional regulators CtrA and GcrA. The cellular levels of CtrA and GcrA are temporally and spatially out-of-phase during the cell cycle, with CtrA repressing gcrA transcription and GcrA activating ctrA transcription. Here, we show that DnaA, a protein required for the initiation of DNA replication, also functions as a transcriptional activator of gcrA, which in turn activates multiple genes, notably those involved in chromosome replication and segregation. The cellular concentration of DnaA is cell cycle-controlled, peaking at the time of replication initiation and gcrA induction. Regulated proteolysis of GcrA contributes to the cell cycle variations in GcrA abundance. We propose that DnaA couples DNA replication initiation with the expression of the two oscillating regulators GcrA and CtrA and that the DnaA/GcrA/CtrA regulatory cascade drives the forward progression of the Caulobacter cell cycle.
摘要
柄杆菌的细胞周期进程由主要转录调节因子CtrA和GcrA驱动。在细胞周期中，CtrA和GcrA的细胞水平在时间和空间上不同步，CtrA抑制gcrA转录，而GcrA激活ctrA转录。在这里，我们表明，DnaA是DNA复制起始所需的一种蛋白质，它还作为gcrA的转录激活因子发挥作用，进而激活多个基因，特别是那些参与染色体复制和分离的基因。DnaA的细胞浓度受细胞周期控制，在复制起始和gcrA诱导时达到峰值。GcrA的调节性蛋白水解作用导致了GcrA丰度的细胞周期变化。我们提出，DnaA将DNA复制起始与两个振荡调节因子GcrA和CtrA的表达联系起来，并且DnaA/GcrA/CtrA调节级联驱动柄杆菌细胞周期的向前推进。
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