用于生物成像的水溶性双光子激发蓝色荧光发色团的合成与表征

Synthesis and characterization of water-soluble two-photon excited blue fluorescent chromophores for bioimaging.

作者信息

Hayek Ali, Bolze Frédéric, Nicoud Jean-François, Baldeck Patrice L, Mély Yves

机构信息

Groupe des Matériaux Organiques, Institut de Physique et Chimie des Matériaux de Strasbourg, CNRS, 23 rue du Loess, F-67034, Strasbourg, France.

出版信息

Photochem Photobiol Sci. 2006 Jan;5(1):102-6. doi: 10.1039/b509843b. Epub 2005 Nov 11.

DOI:10.1039/b509843b

PMID:16395434

Abstract

We report here the synthesis and characterization of a new type of non-ionic blue fluorescent water-soluble chromophores specifically designed for two-photon absorption microscopy. The water solubility is induced by introduction of short oligo(ethylene glycol) monomethyl ether moieties. This work has led to low molecular weight dyes with efficient two-photon absorption cross sections and high fluorescence quantum yield in organic solvents as well as in aqueous solutions.

摘要

我们在此报告一种新型非离子蓝色荧光水溶性发色团的合成与表征，该发色团是专门为双光子吸收显微镜设计的。通过引入短链聚乙二醇单甲醚部分来实现水溶性。这项工作得到了在有机溶剂和水溶液中具有高效双光子吸收截面和高荧光量子产率的低分子量染料。