用于研究动态生物过程的光化学工具。
Photochemical tools to study dynamic biological processes.
作者信息
Specht Alexandre, Bolze Frédéric, Omran Ziad, Nicoud Jean-François, Goeldner Maurice
出版信息
HFSP J. 2009 Aug;3(4):255-64. doi: 10.2976/1.3132954. Epub 2009 May 22.
Abstract
Light-responsive biologically active compounds offer the possibility to study the dynamics of biological processes. Phototriggers and photoswitches have been designed, providing the capability to rapidly cause the initiation of wide range of dynamic biological phenomena. We will discuss, in this article, recent developments in the field of light-triggered chemical tools, specially how two-photon excitation, "caged" fluorophores, and the photoregulation of protein activities in combination with time-resolved x-ray techniques should break new grounds in the understanding of dynamic biological processes.
摘要
光响应生物活性化合物为研究生物过程的动力学提供了可能性。人们已经设计出光触发剂和光开关，能够快速引发各种动态生物现象。在本文中，我们将讨论光触发化学工具领域的最新进展，特别是双光子激发、“笼形”荧光团以及蛋白质活性的光调节与时间分辨X射线技术相结合如何在理解动态生物过程方面开辟新天地。
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