用于双光子激发显微镜的金属离子响应荧光探针
Metal Ion-Responsive Fluorescent Probes for Two-Photon Excitation Microscopy.
作者信息
Sumalekshmy S, Fahrni Christoph J
机构信息
School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332.
出版信息
Chem Mater. 2011;23(3):483-500. doi: 10.1021/cm1021905.
Abstract
Metal ion-responsive fluorescent probes are powerful tools for visualizing labile metal ion pools in live cells. To take full advantage of the benefits offered by two-photon excitation microscopy, including increased depth penetration, reduced phototoxicity, and intrinsic 3D capabilities, the photophysical properties of the probes must be optimized for nonlinear excitation. This review summarizes the challenges associated with the design of two-photon excitable fluorescent probes and labels and offers an overview on recent efforts in developing selective and sensitive reagents for the detection of metal ions in biological systems.
摘要
金属离子响应荧光探针是用于可视化活细胞中不稳定金属离子池的强大工具。为了充分利用双光子激发显微镜所带来的优势，包括增加深度穿透、降低光毒性和固有的三维成像能力，必须针对非线性激发对探针的光物理性质进行优化。本文综述了与双光子可激发荧光探针和标记物设计相关的挑战，并概述了近期在开发用于检测生物系统中金属离子的选择性和灵敏试剂方面所做的努力。
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2
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3
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