与β-环糊精络合的血清素的单光子和多光子激发荧光
Single- and multi-photon excited fluorescence from serotonin complexed with beta-cyclodextrin.
作者信息
Bisby Roger H, Botchway Stanley W, Dad Shakeela, Parker Anthony W
机构信息
Biosciences Research Institute, University of Salford, Salford, UK.
出版信息
Photochem Photobiol Sci. 2006 Jan;5(1):122-5. doi: 10.1039/b508602g. Epub 2005 Nov 11.
The fluorescence of serotonin on binding with beta-cyclodextrin has been studied using both steady state and time-resolved methods. Steady state fluorescence intensity of serotonin at 340 nm showed approximately 30% increase in intensity on binding with K(A) approximately 60 dm(3) mol(-1) and the fluorescence lifetimes showed a corresponding increase. In contrast, the characteristic green fluorescence ('hyperluminescence') of serotonin observed upon multiphoton near-infrared excitation with sub-picosecond pulses was resolved into two lifetime components assigned to free and bound serotonin. The results are of interest in relation to selective imaging and detection of serotonin using the unusual hyperluminescence emission and in respect to recent determinations of serotonin by capillary electrophoresis in the presence of cyclodextrin. The results also suggest that hyperluminescence occurs from multiphoton excitation of a single isolated serotonin molecule.
已使用稳态和时间分辨方法研究了血清素与β-环糊精结合时的荧光。血清素在340nm处的稳态荧光强度显示，与K(A)约为60 dm³ mol⁻¹结合时强度增加约30%，并且荧光寿命相应增加。相比之下，用亚皮秒脉冲进行多光子近红外激发时观察到的血清素特征性绿色荧光（“超荧光”）被解析为两个寿命成分，分别归属于游离和结合的血清素。这些结果对于利用不寻常的超荧光发射进行血清素的选择性成像和检测，以及对于最近在环糊精存在下通过毛细管电泳测定血清素而言具有重要意义。结果还表明，超荧光是由单个分离的血清素分子的多光子激发产生的。
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