Rzaieva O M, Borzova N V, Varbanets' L D, Sokolova O V, Kharkevych O S, Zhdanova N M, Safronova L A
Mikrobiol Z. 2005 Sep-Oct;67(5):19-27.
Screening of producers of alpha-L-rhamnosidase among 692 strains of microorganisms of different taxonomic groups has been performed. A capacity to synthesize the enzyme was revealed in 35.7% of the studied cultures. Representatives of genera Aspergillus, Myrothecium, Penicillium, Eupenicillium (activity 0.1 - 0.335 un./mg of protein) proved to be the most active producers. Complex preparations of alpha-L-rhamnosidase were obtained from cultural liquid of 10 producers by fractionation with ammonium sulfate (30 and 90% saturation); pH- and thermal optimum, as well as pH- and thermostability were analyzed in 7 of them. The Aspergillus, Penicillium, Eupenicillium strains which display high stability and activity under technological values of temperature 20-37 degrees C and medium pH 4.0-6.0 have been chosen for further investigations.
对692株不同分类群的微生物进行了α-L-鼠李糖苷酶产生菌的筛选。在所研究的培养物中，35.7%显示出合成该酶的能力。曲霉属、漆斑菌属、青霉属、真青霉属的代表菌株（活性为0.1 - 0.335单位/毫克蛋白质）被证明是最活跃的产生菌。通过硫酸铵分级分离（饱和度为30%和90%）从10株产生菌的培养液中获得了α-L-鼠李糖苷酶的复合制剂；对其中7种制剂分析了pH和温度最佳值以及pH和热稳定性。已选择在20 - 37摄氏度的工艺温度值和pH 4.0 - 6.0的培养基条件下表现出高稳定性和活性的曲霉属、青霉属、真青霉属菌株进行进一步研究。
