Borzova N V, Malanchuk V M, Barbanets L D, Shkol'nyĭ A T, Sokolova E V, Kharkevich E S, Zhdanova N N, Nagornaia S S
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 2000 Sep-Oct;62(5):3-13.
Screening of producers of alpha-N-acetylgalactosaminidase among 854 strains of micromycetes, 171 yeast and 357 bacterial cultures has been carried out. A capacity to synthesize the enzyme was revealed in 11% of cultures. Representatives of Aspergillus genus (activity--0.11-0.142 un./ml) were most active in producing the enzyme. It has been established that glucosidases spectrum in the culture liquid of 18 most active strains was characterized by complete homogeneity and by the presence of a rather high level (0.5-0.9 un./ml) of alpha-galactosidase activity. Complex preparations of alpha-N-acetylgalactosaminidase and alpha-galactosidase have been obtained from the culture liquid of producers by fractionation by ammonium sulphate (30 and 90% saturation); their pH- and thermo-optimum, pH- and thermal stability have been studied. It was shown possibility to induce alpha-N-acetylgalactosaminidase synthesis by a number of carbohydrates (galactose, glucose, galactosamine, and glucosamine), complex-forming substances (guanidine HCl), nitroaminoguanidin and guanidine carbonate and bovine blood. As a result the strain of Aspergillus niger 185 III was chosen which activity level could be increased more than 3 times (activity--0.6 un./ml as compared to initial one).
对854株微真菌、171株酵母和357株细菌培养物进行了α-N-乙酰半乳糖胺酶产生菌的筛选。在11%的培养物中发现了合成该酶的能力。曲霉属的代表（活性为0.11 - 0.142单位/毫升）在产生该酶方面最为活跃。已确定18株最活跃菌株的培养液中的糖苷酶谱具有完全同质性，且存在相当高水平（0.5 - 0.9单位/毫升）的α-半乳糖苷酶活性。通过硫酸铵分级分离（饱和度为30%和90%）从产生菌的培养液中获得了α-N-乙酰半乳糖胺酶和α-半乳糖苷酶的复合制剂；研究了它们的pH和温度最佳值、pH和热稳定性。结果表明，多种碳水化合物（半乳糖、葡萄糖、半乳糖胺和葡糖胺）、络合物形成物质（盐酸胍）、硝基氨基胍和碳酸胍以及牛血可诱导α-N-乙酰半乳糖胺酶的合成。结果选择了黑曲霉185 III菌株，其活性水平可提高3倍以上（活性与初始相比为0.6单位/毫升）。
