Cotoras Milena, Silva Evelyn
Departamento de Ciencias Biológicas, Facultad de Química y Biología, Universidad de Santiago de Chile.
Mycologia. 2005 Mar-Apr;97(2):485-92. doi: 10.3852/mycologia.97.2.485.
Various stages of the infection process among B. cinerea strains isolated from tomatoes or grapes, belonging to different genetic groups, were compared. It was found that strains of B. cinerea isolated from either grapes or tomatoes showed differences in adhesion patterns and in the percentage of germination on tomato cutin. In strains isolated from tomato the first stage of adhesion occurred faster than in strains isolated from grape. At the same time strains isolated from tomato showed a higher percentage of germination on tomato cutin than the other strains after 9 h of incubation. The production and isoenzymatic patterns of polygalacturonases, pectin methyl esterases, pectin lyases, p-nitrophenylbutyrate esterases and laccases by B. cinerea in solid-state fermentation also were analyzed. Correlation between the production of these enzymes and the origin of the strains was not found. On the other hand all strains produced different isoenzymes and a common pattern between the strains was not observed. The ability of B. cinerea strains to colonize tomato leaves also differs between the isolated strains obtained from grapes and tomato. Strains isolated from tomato were more virulent on tomato leaves than strains isolated from grapes.
对从番茄或葡萄中分离出的、属于不同基因群的灰葡萄孢菌株感染过程的各个阶段进行了比较。结果发现，从葡萄或番茄中分离出的灰葡萄孢菌株在粘附模式和在番茄角质上的萌发率方面存在差异。从番茄中分离出的菌株在番茄角质上的第一阶段粘附比从葡萄中分离出的菌株更快。同时，在培养9小时后，从番茄中分离出的菌株在番茄角质上的萌发率高于其他菌株。还分析了灰葡萄孢在固态发酵中多聚半乳糖醛酸酶、果胶甲基酯酶、果胶裂解酶、对硝基苯丁酸酯酶和漆酶的产生及同工酶模式。未发现这些酶的产生与菌株来源之间的相关性。另一方面，所有菌株产生不同的同工酶，未观察到菌株之间的共同模式。从葡萄和番茄中分离得到的菌株在定殖于番茄叶片的能力上也有所不同。从番茄中分离出的菌株对番茄叶片的致病性比从葡萄中分离出的菌株更强。
