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[运用分子遗传学方法鉴别城市蚊虫致倦库蚊(淡色库蚊骚扰亚种)和溪流库蚊(双翅目:蚊科)]

[Differentiation between the urban mosquito Culex pipiens pipiens F. molestus and Culex torrentium (Diptera: Culicidae) by the molecular genetic methods].

作者信息

Vinogradova E B, Shaĭkevich E V

出版信息

Parazitologiia. 2005 Nov-Dec;39(6):574-6.

Abstract

The molecular characters suitable for the differentiation between the autogenous form of Culex pipiens pipiens (the urban mosquito) and the species Culex torrentium, which was previously identified erroneously as an anautogenous form of C. pipiens pipiens (Shaikevich, Vinogradova, 2004), are given. These mosquitoes differ in the infection by the endosymbiotic bacteria Wolbachia pipientis, 6 transitions among 247 nucleotide sequences of 3' part of the cytochrome oxidase subunit I (COI) gene of mitochondrial DNA (mtDNA), in the mtDNA fragments resulted from the restriction analysis, and in the lengths of second internal transcribed spacer (ITS2) sequences in the ribosomal DNA.

摘要

给出了适合区分尖音库蚊指名亚种(城市蚊虫)的自生型与溪流库蚊的分子特征,溪流库蚊此前被错误鉴定为尖音库蚊指名亚种的非自生型(沙伊克维奇、维诺格拉多娃,2004年)。这些蚊虫在内共生菌嗜虫沃巴赫氏体的感染情况、线粒体DNA(mtDNA)细胞色素氧化酶亚基I(COI)基因3'部分247个核苷酸序列中的6个转换、限制性分析产生的mtDNA片段以及核糖体DNA中第二内部转录间隔区(ITS2)序列的长度方面存在差异。

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