Helisch Armin, Wagner Shawn, Khan Nadeem, Drinane Mary, Wolfram Swen, Heil Matthias, Ziegelhoeffer Tibor, Brandt Ulrike, Pearlman Justin D, Swartz Harold M, Schaper Wolfgang
Department of Experimental Cardiology, Max-Planck-Institute for Physiological & Clinical Research, Bad Nauheim, Germany.
Arterioscler Thromb Vasc Biol. 2006 Mar;26(3):520-6. doi: 10.1161/01.ATV.0000202677.55012.a0. Epub 2006 Jan 5.
To assess the importance of genetic background for collateral artery development.
C57BL/6, BALB/c and 129S2/Sv mice were studied after femoral artery ligation by laser Doppler imaging, visible light oximetry, time-of-flight-magnetic resonance imaging, and treadmill testing; C57BL/6 and BALB/c also underwent electron paramagnetic resonance (EPR) oximetry, x-ray angiography, and histology. C57BL/6 had the least initial distal ischemia and most complete recovery. BALB/c had the most severe initial ischemia and poorest recovery. BALB/c had some vasodilatory reserve in their ligated limbs not seen in the other strains at 3 weeks. By in vivo TOF-magnetic resonance angiography, C57BL/6 had larger preexistent and developed collaterals. By x-ray angiography, C57BL/6 had a higher collateral-dependent filling score and number of visible collaterals immediately after femoral ligation and a higher number of visible collaterals at 1 week but not at 4 weeks. EPR oximetry and histology revealed hypoxia and tissue damage in regions of collateral growth of BALB/c but not C57BL/6 mice. In C57BL/6 BrdUrd uptake in the thigh was limited to larger vessels and isolated perivascular cells. Proliferative activity in collateral arterioles was similar in both strains.
Genetic differences in preexistent collateral vasculature can profoundly affect outcome and milieu for compensatory collateral artery growth after femoral artery occlusion.
评估遗传背景对侧支动脉发育的重要性。
对C57BL/6、BALB/c和129S2/Sv小鼠进行股动脉结扎后，采用激光多普勒成像、可见光血氧测定、飞行时间磁共振成像和跑步机测试进行研究；C57BL/6和BALB/c还进行了电子顺磁共振（EPR）血氧测定、X射线血管造影和组织学检查。C57BL/6最初的远端缺血最少，恢复最完全。BALB/c最初的缺血最严重，恢复最差。BALB/c在其结扎肢体中有一些血管舒张储备，而其他品系在3周时未见。通过体内飞行时间磁共振血管造影，C57BL/6有更大的预先存在的和发育的侧支。通过X射线血管造影，C57BL/6在股动脉结扎后立即有更高的侧支依赖性充盈分数和可见侧支数量，在1周时有更高的可见侧支数量，但在4周时没有。EPR血氧测定和组织学显示BALB/c小鼠侧支生长区域存在缺氧和组织损伤，而C57BL/6小鼠则没有。在C57BL/6中，大腿中的BrdUrd摄取仅限于较大血管和孤立的血管周围细胞。两种品系侧支小动脉的增殖活性相似。
预先存在的侧支血管系统的遗传差异可深刻影响股动脉闭塞后代偿性侧支动脉生长的结果和环境。
