小鼠后肢缺血模型中产后血管生成的评估。

Evaluation of post-natal angiogenesis in a mouse hind limb ischemia model.

作者信息

Recatalá Cristina Arce, Albiero Mattia, Santoro Massimo Mattia

机构信息

Laboratory of Angiogenesis and Cancer Metabolism, DiBio, University of Padua, Padua, Italy; Veneto Institute of Molecular Medicine (VIMM), University of Padova, Padua, Italy.

Department of Medicine (DIMED), University of Padova, Padua, Italy; Veneto Institute of Molecular Medicine (VIMM), University of Padova, Padua, Italy.

出版信息

STAR Protoc. 2023 Apr 17;4(2):102232. doi: 10.1016/j.xpro.2023.102232.

DOI:10.1016/j.xpro.2023.102232

PMID:37071530

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10323016/

Abstract

Hind limb ischemia is a useful model to assess metabolic and cellular responses. Here, we present a protocol for evaluating post-natal angiogenesis in a mouse hind limb ischemia model. We describe steps to induce a severe restriction of blood supply of the femoral artery and vein that mimics the real-life scenario observed in clinical settings. We then detail procedures for follow-up laser Doppler imaging to compare post-ischemic responses of four different mouse strains in their capacity to trigger compensatory arteriogenesis. For complete details on the use and execution of this protocol, please refer to Oberkersch et al. (2022)..

摘要

后肢缺血是评估代谢和细胞反应的有用模型。在此，我们展示了一种在小鼠后肢缺血模型中评估出生后血管生成的方案。我们描述了诱导股动脉和静脉血液供应严重受限的步骤，该步骤模拟了临床环境中观察到的实际情况。然后，我们详细介绍了后续激光多普勒成像的程序，以比较四种不同小鼠品系在触发代偿性动脉生成能力方面的缺血后反应。有关该方案使用和执行的完整详细信息，请参考奥伯克斯等人（2022年）的研究。