Groenendijk C F, Sandbrink J M, Brederode J V, van Damme J M
Department of Plant Ecology and Evolutionary Biology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Heredity (Edinb). 1997 Jan;78(Pt 1):75-83. doi: 10.1038/sj.hdy.6880780.
MtDNA restriction fragment polymorphisms were found between cytoplasmic male-sterility types P and R of Plantago lanceolata with the homologous probe pPl311 and maize mtDNA fragments derived from the regions of atp1, cox1 and cox2. No mtDNA differences were observed between male- sterile and restored plants with the same cytoplasmic type. The con-sistency of the polymorphisms was studied in 83 plants from 24 natural populations in the Netherlands. Within the R-cytoplasm no mtDNA polymorphisms were found, whereas seven variant mtDNA RFLP patterns were observed within the P-cytoplasm using pPl311. We also report on a putative new cytoplasmic male-sterility type, which showed a unique RFLP pattern supporting its distinct status.
利用同源探针pPl311以及源自atp1、cox1和cox2区域的玉米线粒体DNA片段，在披针叶车前草的细胞质雄性不育类型P和R之间发现了线粒体DNA限制性片段多态性。在具有相同细胞质类型的雄性不育和育性恢复植株之间未观察到线粒体DNA差异。对来自荷兰24个自然种群的83株植物的多态性一致性进行了研究。在R细胞质内未发现线粒体DNA多态性，而使用pPl311在P细胞质内观察到了7种变异的线粒体DNA RFLP模式。我们还报道了一种假定的新细胞质雄性不育类型，其显示出独特的RFLP模式，支持其独特的地位。
