Science. 1976 Jul 9;193(4248):158-60. doi: 10.1126/science.193.4248.158.
Mitochondrial DNA from normal and cytoplasmic male-sterile maize was digested with restriction endonucleases RI from Escherichia coli or dIII from Hemophilus influenzae. Electrophoresis of resulting fragments revealed distinctions between the two cytoplasmic types. These distinctions suggest that factors responsible or cytoplasmic male sterility are located in the mitochondrial DNA, and that the mitochondrial genome is not inherited paternally.
用大肠杆菌的限制内切酶 RI 或流感嗜血杆菌的 dIII 消化正常和胞质雄性不育玉米的线粒体 DNA。所得片段的电泳显示两种细胞质类型之间的区别。这些区别表明,导致细胞质雄性不育的因素位于线粒体 DNA 中,并且线粒体基因组不是父系遗传的。