Plasma interleukin-10 and interleukin-12 levels in patients with familial Mediterranean fever.

Familial Mediterranean fever (FMF) is an autosomal recessive disorder characterized by recurrent attacks of fever, polyserositis and arthritis. A vast array of cytokines were analysed in these patients, however, little is known about the pro-inflammatory cytokine interleukin (IL)-12. Plasma IL-12 and IL-10 were measured in 24 patients with FMF (19 active, 5 inactive) and 18 healthy controls by ELISA. From 15 active patients blood was also drawn in attack-free period. Mean plasma IL-12 levels of the FMF patients (mean +/- SEM, 6.84+/-3.59 pg/ml) were higher than the controls (0.13+/-0.09 pg/ml, P < 0.001). Mean IL-12 levels of active (7.02+/-5.23 pg/ml) and inactive patients (6.89+/-5.61 pg/ml) were comparable, and they were higher compared to controls (P < or = 0.001). Mean plasma IL-10 levels of the total FMF patients (3.01+/-1.53 pg/ml) were also higher than the controls (P = 0.024). Patients had higher IL-10 levels in attacks (3.83+/-2.02 pg/ml) compared to levels when they were in remission (1.86+/-1.59 pg/ml, P = 0.046). Significantly elevated IL-12 levels in FMF patients regardless of activity may suggest the presence of a pro-inflammatory state also in the inactive period of FMF. Significant increase in IL-10 levels in FMF group may point to the compensatory suppression of inflammation in active periods of the disease.