Department of Microbiology, Research Center of Barij Essence, 87135-1178 Kashan, Iran.
Phytomedicine. 2010 Aug;17(10):771-4. doi: 10.1016/j.phymed.2010.01.016. Epub 2010 Mar 1.
In this study, we evaluated the antifungal activity of the essential oil from Myrtus communis (myrtle) leaves against Candida albicans (eight clinical isolates and one ATCC type strains) and different species of Aspergillus sp (A. niger, A. parasiticus, six isolates of Aspergillus flavus) using broth micro dilution assay. In addition, we evaluated the synergistic effect between the essential oil and the antifungal compound amphotericin B by checkboard micro titer assay. The essential oil was obtained from myrtle leaves by hydrodistillation method and the oil was analyzed by GC and GC-MS methods. Chemical analysis of oil revealed the presence of 70 components, representing 99.23% of the total oil. 1,8-cineole (36.1%), alpha-pinene (22.5%), linalool (8.4%), bornyl acetate (5.2%), alpha-terpineol (4.4%), linalyl acetate (4.2%) and limonene (3.8%) were found to be the major components of the oil. The antifungal evaluating showed that myrtle oil exhibited good antifungal activity against fungi. Myrtle oil showed significant antifungal activity when combined with amphotericin B.
在这项研究中，我们使用微量肉汤稀释法评估了桃金娘（桃金娘科植物）叶精油对白色念珠菌（八种临床分离株和一株 ATCC 标准株）和不同种类的曲霉菌（黑曲霉、寄生曲霉、六种黄曲霉分离株）的抗真菌活性。此外，我们还通过棋盘微量稀释法评估了精油与抗真菌化合物两性霉素 B 的协同作用。精油通过水蒸馏法从桃金娘叶中提取，并用 GC 和 GC-MS 方法进行分析。油的化学分析表明存在 70 种成分，占总油量的 99.23%。1,8-桉树脑（36.1%）、α-蒎烯（22.5%）、芳樟醇（8.4%）、乙酸龙脑酯（5.2%）、α-松油醇（4.4%）、乙酸芳樟酯（4.2%）和柠檬烯（3.8%）被认为是该油的主要成分。抗真菌评估表明，桃金娘油对真菌表现出良好的抗真菌活性。桃金娘油与两性霉素 B 联合使用时表现出显著的抗真菌活性。
