Söntjens Serge H M, Nettles Dana L, Carnahan Michael A, Setton Lori A, Grinstaff Mark W
Departments of Chemistry and Biomedical Engineering, Boston University, Metcalf Center for Science and Engineering, 590 Commonwealth Avenue, Boston, MA 02215, USA.
Biomacromolecules. 2006 Jan;7(1):310-6. doi: 10.1021/bm050663e.
Photo-crosslinkable dendritic macromolecules are attractive materials for the preparation of cartilage tissue engineering scaffolds that may be optimized for in situ formation of hydrated, mechanically stable, and well-integrated hydrogel scaffolds supporting chondrocytes and chondrogenesis. We designed and synthesized a novel hydrogel scaffold for cartilage repair, based on a multivalent and water-soluble tri-block copolymer consisting of a poly(ethylene glycol) core and methacrylated poly(glycerol succinic acid) dendrimer terminal blocks. The terminal methacrylates allow mild and biocompatible photo-crosslinking with a visible light, facilitating in vivo filling of irregularly shaped defects with the dendrimer-based scaffold. The multivalent dendrimer constituents allow high crosslink densities that inhibit swelling after crosslinking while simultaneously introducing biodegradation sites. The mechanical properties and water content of the hydrogel can easily be tuned by changing the biodendrimer concentration. In vitro chondrocyte encapsulation studies demonstrate significant synthesis of neocartilaginous material, containing proteoglycans and type II collagen.
可光交联的树枝状大分子是制备软骨组织工程支架的有吸引力的材料，这些支架可针对原位形成支持软骨细胞和成软骨作用的水合、机械稳定且良好整合的水凝胶支架进行优化。我们基于由聚乙二醇核心和甲基丙烯酸化聚甘油琥珀酸树枝状聚合物末端嵌段组成的多价水溶性三嵌段共聚物，设计并合成了一种用于软骨修复的新型水凝胶支架。末端甲基丙烯酸酯允许与可见光进行温和且生物相容的光交联，便于用基于树枝状聚合物的支架体内填充不规则形状的缺损。多价树枝状聚合物成分允许高交联密度，这在交联后抑制肿胀，同时引入生物降解位点。通过改变生物树枝状聚合物浓度，可以轻松调节水凝胶的机械性能和含水量。体外软骨细胞包封研究表明，新软骨材料大量合成，其中含有蛋白聚糖和II型胶原蛋白。
