Baig Mukhtiar, Azhar Abid, Zaidi Perveen, Kamal Shahid, Karira Khemomal
Department of Biochemistry, Liaquat College of Medicine and Dentistry, ST-19, Block 15, Gulistan-e-Jauhar, Karachi.
J Coll Physicians Surg Pak. 2005 Dec;15(12):757-60.
To determine the correlation of serum leptin with thyroid hormones in primary hypothyroid male patients and euthyroid lean and obese control subjects.
Comparative study.
Atomic Energy Medical Center, JPMC, Karachi, from 2001 to 2003.
In this study, three male groups were included. Those were 21 newly-diagnosed, untreated hypothyroid male patients (BMI, 25.12+/-2.31), 24 age and BMI matched euthyroid lean male subjects (BMI, 24.01+/-1.97) and 27 euthyroid obese male subjects (BMI, 33.02+/-2.67). Patients were selected from the thyroid OPD of Atomic Energy Medical Centre, JPMC, Karachi, while healthy, age and BMI matched euthyroid (lean and obese) were selected from general population after checking their thyroid profile. Patients and control subjects suffering from diabetes or other endocrinal diseases and taking thyroxin or steroid were excluded from the study. Serum leptin was measured by ELISA and FT4, FT3 and TSH were measured by radioimmunoassay (RIA), triacylglycerol (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) by kit method, low density lipoprotein cholesterol (LDL-C) was calculated by the Friedwald s formula, and fasting blood sugar (FBS) by glucose oxidase method.
The mean +/- SEM values of leptin in male hypothyroid patients were 10.71+/-2.5 ng/ml, 8.27+/-1.91 in control group and 21.34+/-3.4 ng/ml in obese group, respectively. No significant difference was found in serum leptin levels between hypothyroid patients and their age, BMI matched control group, while obese control group had significantly higher values of leptin (<0.05). There was no significant correlation of leptin found with T4, T3, and TSH in hypothyroid patients and lean and obese control subjects. Triacylglycerol (TG, p < 0.005), total cholesterol (TC, p < 0.005) and low density lipoprotein cholesterol (LDL-C, p < 0.05) were significantly higher in hypothyroid patients as compared to control groups. This study observed that serum leptin level was significantly correlated with the BMI (r = 0.732, p < 0.005, r = 0.783, p < 0.005, r = 0.653, p < 0.005), in normal lean, obese and hypothyroid male patients respectively.
The results of this study suggest that there is no correlation between serum leptin and thyroid hormones in hypothyroid patients as well as in euthyroid subjects. Serum leptin is directly related with BMI.
确定原发性甲状腺功能减退男性患者以及甲状腺功能正常的消瘦和肥胖对照者血清瘦素与甲状腺激素之间的相关性。
对比研究。
2001年至2003年，卡拉奇真纳医学院核医学中心。
本研究纳入三组男性。分别为21例新诊断的未经治疗的甲状腺功能减退男性患者（BMI，25.12±2.31）、24例年龄及BMI匹配的甲状腺功能正常的消瘦男性受试者（BMI，24.01±1.97）和27例甲状腺功能正常的肥胖男性受试者（BMI，33.02±2.67）。患者选自卡拉奇真纳医学院核医学中心甲状腺门诊，而健康的、年龄及BMI匹配的甲状腺功能正常者（消瘦和肥胖）在检查甲状腺状况后从普通人群中选取。患有糖尿病或其他内分泌疾病以及正在服用甲状腺素或类固醇的患者和对照者被排除在研究之外。采用酶联免疫吸附测定法（ELISA）测定血清瘦素，采用放射免疫分析法（RIA）测定游离甲状腺素（FT4）、游离三碘甲状腺原氨酸（FT3）和促甲状腺激素（TSH），采用试剂盒法测定三酰甘油（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C），采用Friedwald公式计算低密度脂蛋白胆固醇（LDL-C），采用葡萄糖氧化酶法测定空腹血糖（FBS）。
甲状腺功能减退男性患者瘦素的平均±标准误值分别为10.71±2.5 ng/ml，对照组为8.27±1.91 ng/ml，肥胖组为21.34±3.4 ng/ml。甲状腺功能减退患者与其年龄、BMI匹配的对照组之间血清瘦素水平无显著差异，而肥胖对照组的瘦素值显著更高（<0.05）。在甲状腺功能减退患者以及消瘦和肥胖对照者中，未发现瘦素与T4、T3和TSH之间存在显著相关性。与对照组相比，甲状腺功能减退患者的三酰甘油（TG，p<0.005）、总胆固醇（TC，p<0.005）和低密度脂蛋白胆固醇（LDL-C，p<0.05）显著更高。本研究观察到，在正常消瘦、肥胖和甲状腺功能减退男性患者中，血清瘦素水平分别与BMI显著相关（r = 0.732，p<0.005；r = 0.783，p<0.005；r = 0.653，p<0.005）。
本研究结果表明，甲状腺功能减退患者以及甲状腺功能正常者的血清瘦素与甲状腺激素之间无相关性。血清瘦素与BMI直接相关。
