Mirza Sajjad Hussain, Rehman Habib ur, Khan Mumtaz Ahmad
Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi.
J Coll Physicians Surg Pak. 2005 Dec;15(12):771-3.
The objective of this study was to find out the prevalence of Pityrosporum species in the patients with seborrheic dermatitis (SD) and compare the colonization rate with normal healthy individuals.
Comparative study.
Dermatology Department, Military Hospital and Department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi from 01 March 1996 to 28 February 1997.
Fifty patients with clinically diagnosed seborrheic dermatitis were included in this study. Fifty normal healthy individual with matched age and gender were included as control subjects. Three samples from each effected sites were taken from 2 cm2 area. Identical sites in control subjects were also sampled. These samples were examined under the microscope after treating with 10% potassium hydroxide and staining with parker blue black ink for the presence of Pityrosporum yeast cells. The specimen were also inoculated in special lipid enriched media, incubated at 37 degrees C for 4-6 days.
Out of 50 patients, Pityrosporum yeast cells were seen microscopically in 37 (74%), and the cultures were positive in 43 (86%). Among the normal individuals yeast cells were seen microscopically in 23 (46%) and the cultures were positive in 22 (44%).
The colonization rate of Pityrosporum species was higher in the seborrheic dermatitis patients. It might be playing a causative role in the aetiology of this disease.
本研究的目的是查明脂溢性皮炎（SD）患者中马拉色菌属的患病率，并将其定植率与正常健康个体进行比较。
对比研究。
1996年3月1日至1997年2月28日，拉瓦尔品第军事医院皮肤科及武装部队病理研究所微生物科。
本研究纳入50例临床诊断为脂溢性皮炎的患者。纳入50名年龄和性别匹配的正常健康个体作为对照。从每个患病部位2平方厘米区域采集三个样本。对照个体的相同部位也进行采样。这些样本用10%氢氧化钾处理并用派克蓝黑墨水染色后在显微镜下检查马拉色菌酵母细胞的存在情况。标本还接种于特殊的富含脂质的培养基中，在37℃孵育4 - 6天。
50例患者中，显微镜下可见马拉色菌酵母细胞的有37例（74%），培养阳性的有43例（86%）。正常个体中显微镜下可见酵母细胞的有23例（46%），培养阳性的有22例（44%）。
脂溢性皮炎患者中马拉色菌属的定植率较高。它可能在该疾病的病因学中起致病作用。
