Cantafora A, Masella R
Laboratory of Metabolism and Pathological Biochemistry, Istituto Superiore di Sanità, Rome, Italy.
J Chromatogr. 1992 Feb 28;593(1-2):139-46. doi: 10.1016/0021-9673(92)80278-3.
Phosphatidylcholine isolated from samples of bile, liver and plasma was converted into 1,2-diradylglycerobenzoate molecular species by hydrolysis with phospholipase C and reaction with benzoic anhydride. Up to seventeen molecular species were separated and determined by reversed-phase high-performance liquid chromatography with detection at 230 nm. The major improvement introduced here was the use of distearoylphosphatidylcholine as the internal standard, which corrected the results for incomplete hydrolysis and benzoylation. Other improvements concerned the clean-up of benzoyl derivatives and the chromatographic separation. The analytical results obtained were validated by comparison with the results of either lipid phosphorus or gas chromatographic determinations.
从胆汁、肝脏和血浆样本中分离出的磷脂酰胆碱，通过磷脂酶C水解并与苯甲酸酐反应，转化为1,2 - 二酰基甘油苯甲酸酯分子种类。通过反相高效液相色谱在230 nm处检测，分离并测定了多达十七种分子种类。此处引入的主要改进是使用二硬脂酰磷脂酰胆碱作为内标，它校正了水解和苯甲酰化不完全的结果。其他改进涉及苯甲酰衍生物的净化和色谱分离。通过与脂质磷或气相色谱测定结果进行比较，验证了所获得的分析结果。
