Cantafora A, Di Biase A, Alvaro D, Angelico M, Marin M, Attili A F
Clin Chim Acta. 1983 Nov 15;134(3):281-95. doi: 10.1016/0009-8981(83)90367-4.
In this paper we propose a novel, rapid and simple high-performance liquid chromatographic (HPLC) method for the identification and quantitation of individual phosphatidylcholine (PC) molecular species from natural mixtures. To overcome difficulties deriving from the lack of adequate standards and from the variability of the responses to UV spectrophotometric detectors currently used in HPLC analysis, we first fractionated and quantitated the major molecular species of a commercial egg PC by means of a preparative column. The identification of PC molecular species was confirmed by gas-liquid chromatographic analysis of fatty acids. We employed the fractions recovered from preparative HPLC to determine the detector calibration factors of the individual molecular species separated using an analytical, high-speed, reversed-phase HPLC column. The proposed method seems to be adequate for the analysis of PC from many biological sources. Its application to the analysis of human hepatic and gallbladder biliary PC is shown.
在本文中，我们提出了一种新颖、快速且简单的高效液相色谱（HPLC）方法，用于从天然混合物中鉴定和定量单个磷脂酰胆碱（PC）分子种类。为克服因缺乏合适标准品以及当前HPLC分析中使用的紫外分光光度检测器响应变异性所带来的困难，我们首先通过制备柱对市售鸡蛋PC的主要分子种类进行了分离和定量。通过脂肪酸的气液色谱分析确认了PC分子种类的鉴定。我们使用从制备型HPLC回收的馏分来确定使用分析型、高速反相HPLC柱分离的各个分子种类的检测器校准因子。所提出的方法似乎适用于分析来自多种生物来源的PC。展示了其在人肝和胆囊胆汁PC分析中的应用。
