Del Nero S, Di Somma M, Vigevani A
Department of Analytical Chemistry, R & D Farmitalia Carlo Erba Srl, Milan, Italy.
J Chromatogr. 1992 Feb 28;593(1-2):25-8. doi: 10.1016/0021-9673(92)80261-r.
The cytochrome P-450-dependent aromatase enzyme plays an important role in hormone-dependent diseases. Many products that inhibit this type of enzyme were obtained: FCE 24304 (I) and FCE 24928 (II) proved to possess remarkable activity and are presently under development. Compounds I and II and their synthetic intermediates are analyzed by means of a high-performance liquid chromatographic method, affording rapid and efficient separation, good resolution and identification of all the examined compounds. The linearity, specificity, sensitivity, precision and accuracy for the method are also provided.
细胞色素P - 450依赖性芳香化酶在激素依赖性疾病中起重要作用。已获得许多抑制这类酶的产物：FCE 24304（I）和FCE 24928（II）被证明具有显著活性，目前正在研发中。通过高效液相色谱法对化合物I和II及其合成中间体进行分析，能快速有效地分离，所有检测化合物都具有良好的分离度和鉴别能力。还给出了该方法的线性、特异性、灵敏度、精密度和准确度。
