高效液相色谱法测定人血浆中新型芳香酶抑制剂FCE 24928

High-performance liquid chromatographic determination of FCE 24928, a new aromatase inhibitor, in human plasma.

作者信息

Fontana E, Pianezzola E, Basileo G, Strolin Benedetti M

机构信息

Farmitalia Carlo Erba, Erbamont Group, Milan, Italy.

出版信息

J Chromatogr A. 1994 Feb 4;660(1-2):293-8. doi: 10.1016/0021-9673(94)85124-7.

DOI:10.1016/0021-9673(94)85124-7

PMID:8148992

Abstract

A sensitive and selective high-performance liquid chromatographic method for the determination of FCE 24928 (4-amino-androsta-1,4,6-triene-3,17-dione) in human plasma is reported. The drug was extracted from buffered (pH = 8) plasma samples with methylene chloride-isooctane, then analysed by reversed-phase liquid chromatography. Quantitation was achieved by ultraviolet detection of the eluate at 238 nm. Blank plasma samples from humans, dog and rat assayed as described showed no significant peak at the retention time of the compound of interest. The suitability of the method for in vivo samples was tested by measuring the plasma levels of FCE 24928 in rats that received oral doses of the test compound.

摘要

报道了一种灵敏且具选择性的高效液相色谱法，用于测定人血浆中的FCE 24928（4-氨基-雄甾-1,4,6-三烯-3,17-二酮）。该药物用二氯甲烷-异辛烷从缓冲（pH = 8）血浆样品中萃取，然后通过反相液相色谱进行分析。通过在238 nm处对洗脱液进行紫外检测来实现定量。按所述方法测定的来自人、狗和大鼠的空白血浆样品在目标化合物的保留时间处未显示出明显峰。通过测量口服给予受试化合物的大鼠血浆中FCE 24928的水平，测试了该方法对体内样品的适用性。