Hosseinzadeh Hossein, Sadeghnia Hamid R, Ziaee Toktam, Danaee Aghdas
Pharmaceutical Research Center, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran.
J Pharm Pharm Sci. 2005 Aug 22;8(3):387-93.
The generation of reactive oxygen species and lipid peroxidation are associated with tissue injury following ischemic insult; therefore, the use of antioxidants appears rational in the improvement of kidney diseases therapy. The aim of the present study was to assess the effect of aqueous saffron extract (Crocus sativus L.) and its active constituent, crocin, on oxidative stress following renal ischemia-reperfusion injury (IRI) in rats.
The cellular redox status (thiobarbituric acid reactive species (TBARS) and total thiol levels) and antioxidant power (using ferric reducing/antioxidant power test) were assessed in control and ischemic groups. The left kidney was exposed to warm ischemia for 60 min followed by reperfusion for 90 min. The macerated aqueous extract of saffron (with doses of 5, 20 and 80 mg/kg, i.p.) and crocin (with doses of 50, 200 and 400 mg/kg, i.p.) were administrated prior to induction of ischemia. Normal saline (10 ml/kg, i.p.) was injected to control group and a sham group that did not have ischemia-reperfusion.
Ischemia-reperfusion (IR) caused a significant increase in TBARS levels (p<0.001) and decrement in both antioxidant power (FRAP value) (p<0.05) and total thiol concentration (p<0.001) in kidney homogenate samples. In crocin pretreated groups, a reduction in TBARS levels (from 85.8 +/- 5.4 to 20.9 +/- 1.5 nmol/g tissue, p<0.001; 400 mg/kg) and elevation in antioxidant power (FRAP value) (from 3.05 +/- 0.16 to 4.15 +/- 0.16 micromol/g tissue, p<0.001; 400 mg/kg) and total thiol concentrations (from 0.38 +/- 0.03 to 0.62 +/- 0.03 mM, p<0.001; 200 mg/kg), as compared with control group, were observed. The aqueous extract also reduced lipid peroxidation products (from 85.8 +/- 5.4 to 15.9 +/- 2.6 nmol/g tissue, p<0.001; 80 mg/kg) and increased antioxidant power (from 2.98 +/- 0.11 to 5.97 +/- 0.56 micromol/g tissue, p<0.001; 80 mg/kg) in ischemia-reperfusion injured rat kidneys.
This study therefore suggests that the aqueous saffron extract (Crocus sativus L.) and its active constituent, crocin, may be useful agents for the prevention of renal ischemia-reperfusion (IR)-induced oxidative injury in rats.
活性氧的产生和脂质过氧化与缺血性损伤后的组织损伤相关；因此，使用抗氧化剂似乎是改善肾脏疾病治疗的合理方法。本研究的目的是评估藏红花水提取物（藏红花）及其活性成分西红花苷对大鼠肾缺血再灌注损伤（IRI）后氧化应激的影响。
在对照组和缺血组中评估细胞氧化还原状态（硫代巴比妥酸反应性物质（TBARS）和总硫醇水平）和抗氧化能力（使用铁还原/抗氧化能力测试）。左肾暴露于热缺血60分钟，随后再灌注90分钟。在缺血诱导前给予藏红花的浸软水提取物（剂量为5、20和80mg/kg，腹腔注射）和西红花苷（剂量为50、200和400mg/kg，腹腔注射）。向对照组和未进行缺血再灌注的假手术组注射生理盐水（10ml/kg，腹腔注射）。
缺血再灌注（IR）导致肾脏匀浆样品中TBARS水平显著升高（p<0.001），抗氧化能力（FRAP值）降低（p<0.05）和总硫醇浓度降低（p<0.001）。在西红花苷预处理组中，与对照组相比，观察到TBARS水平降低（从85.8±5.4降至20.9±1.5nmol/g组织，p<0.001；400mg/kg），抗氧化能力（FRAP值）升高（从3.05±0.16升至4.15±0.16μmol/g组织，p<0.001；400mg/kg）和总硫醇浓度升高（从0.38±0.03升至0.62±0.03mM，p<0.001；200mg/kg）。水提取物还降低了缺血再灌注损伤大鼠肾脏中的脂质过氧化产物（从85.8±5.4降至15.9±2.6nmol/g组织，p<0.001；80mg/kg），并提高了抗氧化能力（从2.98±0.11升至5.97±0.56μmol/g组织，p<0.001；80mg/kg）。
因此，本研究表明藏红花水提取物（藏红花）及其活性成分西红花苷可能是预防大鼠肾缺血再灌注（IR）诱导的氧化损伤的有用药物。
