Hwang Ji Hye, Ra Yun-Ju, Lee Kyung Mu, Lee Ji Youl, Ghil Sung Ho
Department of Physical Medicine and Rehabilitation, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Republic of Korea.
Arch Phys Med Rehabil. 2006 Jan;87(1):20-6. doi: 10.1016/j.apmr.2005.08.002.
To evaluate the muscle healing effect of passive mobilization exercises after a laceration injury.
Randomized controlled trial.
Basic science laboratory.
Male Sprague-Dawley rats (N=36), age ranging from 8 to 10 weeks and weight ranging from 300 to 400 g.
The bilateral gastrocnemius muscles were lacerated. The left leg muscles were used as the study groups and the right side was used as the control (lacerated muscles without any treatment, n=8). In the exercise group (n=24), passive mobilization exercise (15 min/d) was performed for 5 days starting from different time points (2, 7, and 14d postlaceration). The decorin group (n=8) was injected with decorin (50 microg at 14d postlaceration), which is a well-known antifibrotic agent. Four animals were used as the normal controls, in which only the muscle strength was evaluated. All the animals were killed 4 weeks after the laceration.
The histologic characterization of muscle regeneration (hematoxylin and eosin staining, number and diameter of the centronucleated, regenerating myofibers), muscle fibrosis (vimentin-positive area, Masson modified trichrome staining positive area), and muscle strength (analysis of fast twitch strength).
The level of fibrosis was more than 50% lower in the exercise and decorin groups than in the control (P<.05). The decorin group showed the highest number of regenerated, new myofibers and the highest muscle strength. All of the exercise groups, regardless of the starting time of exercise, also showed significant improvement in regeneration and strength. However, the exercise group starting 14 days after the laceration showed the best results.
Stretching exercises after a muscle laceration injury has a strong antifibrotic effect, as much as a well-known antifibrotic agent, decorin. According to the results, the best time to begin stretching exercises is 14 days after laceration for antifibrosis and muscle regeneration.
评估撕裂伤后被动活动锻炼对肌肉愈合的效果。
随机对照试验。
基础科学实验室。
雄性斯普拉格 - 道利大鼠（N = 36），年龄8至10周，体重300至400克。
双侧腓肠肌进行撕裂伤处理。左腿肌肉作为研究组，右侧作为对照组（撕裂伤肌肉不做任何处理，n = 8）。运动组（n = 24）从不同时间点（撕裂伤后2、7和14天）开始，进行为期5天的被动活动锻炼（每天15分钟）。核心蛋白聚糖组（n = 8）在撕裂伤后14天注射核心蛋白聚糖（50微克），核心蛋白聚糖是一种知名的抗纤维化剂。4只动物作为正常对照组，仅评估肌肉力量。所有动物在撕裂伤后4周处死。
肌肉再生的组织学特征（苏木精和伊红染色、中央核化再生肌纤维的数量和直径）、肌肉纤维化（波形蛋白阳性区域、改良马松三色染色阳性区域）和肌肉力量（快肌力量分析）。
运动组和核心蛋白聚糖组的纤维化水平比对照组低50%以上（P <.05）。核心蛋白聚糖组再生的新肌纤维数量最多，肌肉力量最强。所有运动组，无论运动开始时间如何，在再生和力量方面也都有显著改善。然而，撕裂伤后14天开始运动的运动组效果最佳。
肌肉撕裂伤后进行拉伸锻炼具有很强的抗纤维化作用，与知名抗纤维化剂核心蛋白聚糖的作用相当。根据结果，开始拉伸锻炼的最佳时间是撕裂伤后14天，以实现抗纤维化和肌肉再生。
