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Difluorotoluene, a Nonpolar Isostere for Thymine, Codes Specifically and Efficiently for Adenine in DNA Replication.二氟甲苯，胸腺嘧啶的一种非极性生物电子等排体，在DNA复制中特异性且高效地编码腺嘌呤。
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硫代胸苷（胸苷的扩展大小变体）的碱基配对增强及复制效率提高。

Enhanced base pairing and replication efficiency of thiothymidines, expanded-size variants of thymidine.

作者信息

Sintim Herman O, Kool Eric T

机构信息

Department of Chemistry, Stanford University, California 94305-5080, USA.

出版信息

J Am Chem Soc. 2006 Jan 18;128(2):396-7. doi: 10.1021/ja0562447.

DOI:10.1021/ja0562447
PMID:16402811
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2555968/
Abstract

Here we test the steric effects of added size on DNA base pairing and replication by the use of thiocarbonyl group replacements for natural nucleoside thymidine. The 2-thioT (2S) and 4-thioT (4S) nucleosides were reported previously, but their pairing specificity and replication fidelity were unknown. We find that 2S pairs with higher specificity than thymidine, and both 2S and 4S nucleoside triphosphates are replicated with higher efficiency than natural dTTP. The results indicate possible biotechnological uses for these analogues and have implications in the ongoing use of thionucleobases in cancer treatment.

摘要

在这里，我们通过使用硫代羰基取代天然核苷胸腺嘧啶来测试添加的大小对DNA碱基配对和复制的空间效应。2-硫代胸腺嘧啶（2S）和4-硫代胸腺嘧啶（4S）核苷此前已有报道，但其配对特异性和复制保真度尚不清楚。我们发现，2S的配对特异性高于胸腺嘧啶，并且2S和4S核苷三磷酸的复制效率均高于天然的三磷酸胸苷（dTTP）。这些结果表明这些类似物可能具有生物技术用途，并且对硫代碱基在癌症治疗中的持续应用具有启示意义。