一种比率型荧光粘度传感器。

A ratiometric fluorescent viscosity sensor.

作者信息

Haidekker Mark A, Brady Thomas P, Lichlyter Darcy, Theodorakis Emmanuel A

机构信息

Department of Biological Engineering, University of Missouri--Columbia, 65211, USA.

出版信息

J Am Chem Soc. 2006 Jan 18;128(2):398-9. doi: 10.1021/ja056370a.

DOI:10.1021/ja056370a

PMID:16402812

Abstract

The development of a dual probe that provides ratiometric measurements of fluid viscosity is described. The design is based on coupling of a primary fluorophore with viscosity-independent fluorescence emission (blue unit) with a secondary fluorophore that exhibits viscosity-sensitive fluorescent emission quantum yield (red unit). Excitation of the secondary fluorophore can be achieved via Resonance Energy Transfer. The ratio of the fluorescence emission of these fluorophores provides an accurate, ratiometric measurement of solvent viscosity.

摘要

本文描述了一种用于流体粘度比率测量的双探针的开发。该设计基于将具有与粘度无关的荧光发射的初级荧光团（蓝色单元）与表现出粘度敏感荧光发射量子产率的次级荧光团（红色单元）耦合。次级荧光团的激发可通过共振能量转移实现。这些荧光团的荧光发射比率提供了溶剂粘度的准确比率测量。