用一种诊疗用铼配合物定量测定免疫原性细胞死亡过程中的内质网粘度。

Quantitative Determination of Endoplasmic Reticulum Viscosity during Immunogenic Cell Death by a Theranostic Rhenium Complex.

作者信息

Chen Xiao-Xiao, Rao Xing-Yi, Guan Qi-Xin, Wang Peng, Tan Cai-Ping

机构信息

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, P. R. China.

出版信息

Chem Biomed Imaging. 2023 Oct 12;2(1):64-69. doi: 10.1021/cbmi.3c00084. eCollection 2024 Jan 22.

DOI:10.1021/cbmi.3c00084

PMID:39473464

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11504626/

Abstract

The endoplasmic reticulum (ER) is an important targeting organelle for metal-based immunogenic cell death (ICD) inducers. Metal complexes can induce ER stress by causing protein misfolding, which can be reflected by alternations in microenvironmental parameters, including viscosity. We present here a theranostic Re(I) complex () that shows viscosity-dependent emission intensity and lifetime. can trigger immunogenic cell death (ICD) in MDA-MB-231 cells by localizing in the ER and causing ER stress. We demonstrate that can simultaneously induce and monitor the gradual increase in the ER viscosity quantitatively.

摘要

内质网（ER）是金属基免疫原性细胞死亡（ICD）诱导剂的重要靶向细胞器。金属络合物可通过导致蛋白质错误折叠来诱导内质网应激，这可通过包括粘度在内的微环境参数的变化反映出来。我们在此展示了一种诊疗用铼（I）络合物（），其发射强度和寿命呈现粘度依赖性。该络合物可定位于内质网并引起内质网应激，从而在MDA-MB-231细胞中触发免疫原性细胞死亡（ICD）。我们证明该络合物能够同时定量诱导并监测内质网粘度的逐渐增加。