Fischer Derek, Theodorakis Emmanuel A, Haidekker Mark A
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA.
Nat Protoc. 2007;2(1):227-36. doi: 10.1038/nprot.2006.455.
A procedure for the synthesis of a ratiometric viscosity fluorescent sensor is described in this protocol. The essential requirement for the design of this sensor is the attachment of a primary fluorophore that has both a viscosity-independent fluorescence emission (coumarin dye shown in blue) and an emission from a fluorophore that exhibits viscosity-dependent fluorescent quantum yield (p-amino cinnamonitrile dye shown in red). The use of sensor 1 in viscosity measurements involves solubilization in a liquid of interest and excitation of the primary fluorophore at lambda(ex) = 360 nm. The secondary fluorophore is simultaneously excited via resonance energy transfer. The ratio of the fluorescent emission of the secondary over the primary fluorophore provides a fast and precise measurement of the viscosity of the solvent. The synthesis of compound 1 using commercially available materials can be completed within 5 d.
本方案描述了一种用于合成比率型粘度荧光传感器的方法。该传感器设计的基本要求是连接一个具有与粘度无关的荧光发射（蓝色显示的香豆素染料）的初级荧光团和一个来自荧光团的发射，该荧光团表现出与粘度相关的荧光量子产率（红色显示的对氨基肉桂腈染料）。在粘度测量中使用传感器1涉及将其溶解在感兴趣的液体中，并在λ(ex)=360nm处激发初级荧光团。次级荧光团通过共振能量转移同时被激发。次级荧光团与初级荧光团的荧光发射比率提供了对溶剂粘度的快速而精确的测量。使用市售材料合成化合物1可在5天内完成。
