II型糖尿病患者前β1高密度脂蛋白（HDL）和α高密度脂蛋白的动力学

Kinetics of prebeta1 HDL and alphaHDL in type II diabetic patients.

作者信息

Chétiveaux M, Lalanne F, Lambert G, Zair Y, Ouguerram K, Krempf M

机构信息

Inserm U539, Centre de Recherche en Nutrition Humaine, CHU Hôtel Dieu, Nantes, France.

出版信息

Eur J Clin Invest. 2006 Jan;36(1):29-34. doi: 10.1111/j.1365-2362.2006.01586.x.

DOI:10.1111/j.1365-2362.2006.01586.x

PMID:16403007

Abstract

BACKGROUND

The aim of this study was to analyze the recycling of high density lipoprotein (HDL) in six type II diabetic patients compared with six control subjects by endogenous labelling of apolipoprotein A-I (Apo A-I) with stable isotope Apo A.

MATERIALS AND METHODS

The -I-HDL kinetics were performed by infusion of (5.5.5-(2)H3)-leucine for 14 h. The prebeta1 and alphaHDL were separated by gel filtration fast protein liquid chromatrography system (FPLC). Kinetics of isotopic enrichment of Apo A-I were analyzed with a multi-compartmental model software (SAAM II, SAAM Institute, Seattle, WA).

RESULTS

Plasma Apo A-I concentration was decreased in patients with type II diabetes as a result of a decrease in Apo A-I-alphaHDL (P < 0.05). Diabetic patients were also characterized by an increased relative contribution of Apo A-I in prebeta1 HDL (18.3 +/- 2.8% vs 11.9 +/- 3.7%, P < 0.01). The synthetic rate of prebeta1 HDL was slightly increased in diabetic patients compared with control (NS) and an increase of recycling rate of alpha to prebeta1 HDL was observed (11.67 +/- 3.14 d(-1) vs 7.09 +/- 4.51 d(-1), P < 0.05). The clearance rate of Apo A-I was higher in diabetic patients (P < 0.05 for Apo A-I-prebeta1 HDL and P < 0.005 for Apo A-I-alphaHDL).

CONCLUSION

This study suggests that the usual increase in prebeta1 HDL in type II diabetic patients is mainly related to an increased conversion rate of alpha to prebeta1 HDL.

摘要

背景

本研究旨在通过用稳定同位素载脂蛋白A（Apo A）对内源性载脂蛋白A-I（Apo A-I）进行标记，分析6例II型糖尿病患者与6例对照者中高密度脂蛋白（HDL）的再循环情况。

材料与方法

通过输注（5,5,5-（2）H3）-亮氨酸14小时来进行载脂蛋白-I-高密度脂蛋白（-I-HDL）动力学研究。通过凝胶过滤快速蛋白质液相色谱系统（FPLC）分离前β1和αHDL。使用多室模型软件（SAAM II，华盛顿州西雅图市SAAM研究所）分析Apo A-I的同位素富集动力学。

结果

II型糖尿病患者血浆Apo A-I浓度因Apo A-I-αHDL降低而下降（P < 0.05）。糖尿病患者的特征还包括Apo A-I在前β1 HDL中的相对贡献增加（18.3±2.8% 对11.9±3.7%，P < 0.01）。与对照组相比，糖尿病患者前β1 HDL的合成率略有增加（无统计学差异），并且观察到αHDL向前β1 HDL的再循环率增加（11.67±3.14 d-1对7.09±4.51 d-1，P < 0.05）。糖尿病患者中Apo A-I的清除率更高（Apo A-I-前β1 HDL的P < 0.05，Apo A-I-αHDL的P < 0.005）。