Wuhrer Manfred, Koeleman Carolien A M, Deelder André M, Hokke Cornelis H
Biomolecular Mass Spectrometry Unit, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.
FEBS J. 2006 Jan;273(2):347-61. doi: 10.1111/j.1742-4658.2005.05068.x.
N-Glycans from glycoproteins of the worm stage of the human parasite Schistosoma mansoni were enzymatically released, fluorescently labelled and analysed using various mass spectrometric and chromatographic methods. A family of 28 mainly core-alpha1-6-fucosylated, diantennary N-glycans of composition Hex(3-4)HexNAc(6-12)Fuc(1-6) was found to carry dimers of N,N'-diacetyllactosediamine [LacdiNAc or LDN; GalNAc(beta1-4)GlcNAc(beta1-] with or without fucose alpha1-3-linked to the N-acetylglucosamine residues in the antennae {GalNAc(beta1-4)[+/-Fuc(alpha1-3)]GlcNAc(beta1-3)GalNAc(beta1-4)[+/-Fuc(alpha1-3)]GlcNAc(beta1-}. To date, oligomeric LDN and oligomeric fucosylated LDN (LDNF) have been found only on N-glycans from mammalian cells engineered to express Caenorhabditis elegansbeta4-GalNAc transferase and human alpha3-fucosyltransferase IX [Z. S. Kawar et al. (2005) J Biol Chem280, 12810-12819]. It now appears that LDN(F) repeats can also occur in a natural system such as the schistosome parasite. Like monomeric LDN and LDNF, the dimeric LDN(F) moieties found here are expected to be targets of humoral and cellular immune responses during schistosome infection.
从人类寄生虫曼氏血吸虫蠕虫阶段糖蛋白中酶解释放出N -聚糖，进行荧光标记，并使用各种质谱和色谱方法进行分析。发现了一个由28种主要为核心α1 - 6 -岩藻糖基化的双天线N -聚糖组成的家族，其组成为Hex(3 - 4)HexNAc(6 - 12)Fuc(1 - 6)，带有N,N'-二乙酰乳糖二胺[LacdiNAc或LDN；GalNAc(β1 - 4)GlcNAc(β1 - ]的二聚体，天线中的N - 乙酰葡糖胺残基上有或没有α1 - 3连接的岩藻糖{GalNAc(β1 - 4)[+/-Fuc(α1 - 3)]GlcNAc(β1 - 3)GalNAc(β1 - 4)[+/-Fuc(α1 - 3)]GlcNAc(β1 - }。迄今为止，仅在经过基因工程改造以表达秀丽隐杆线虫β4 - GalNAc转移酶和人类α3 - 岩藻糖基转移酶IX的哺乳动物细胞的N -聚糖上发现了寡聚LDN和寡聚岩藻糖基化LDN（LDNF）[Z. S. Kawar等人（2005年）《生物化学杂志》280卷，12810 - 128。现在看来，LDN(F)重复序列也可以出现在诸如血吸虫寄生虫这样的天然系统中。与单体LDN和LDNF一样，这里发现的二聚体LDN(F)部分预计在血吸虫感染期间是体液和细胞免疫反应的靶点。
