Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, UK.
J Am Soc Mass Spectrom. 2018 Jun;29(6):1179-1193. doi: 10.1007/s13361-018-1950-x. Epub 2018 May 22.
There is considerable potential for the use of ion mobility mass spectrometry in structural glycobiology due in large part to the gas-phase separation attributes not typically observed by orthogonal methods. Here, we evaluate the capability of traveling wave ion mobility combined with negative ion collision-induced dissociation to provide structural information on N-linked glycans containing multiple fucose residues forming the Lewis and Lewis epitopes. These epitopes are involved in processes such as cell-cell recognition and are important as cancer biomarkers. Specific information that could be obtained from the intact N-glycans by negative ion CID included the general topology of the glycan such as the presence or absence of a bisecting GlcNAc residue and the branching pattern of the triantennary glycans. Information on the location of the fucose residues was also readily obtainable from ions specific to each antenna. Some isobaric fragment ions produced prior to ion mobility could subsequently be separated and, in some cases, provided additional valuable structural information that was missing from the CID spectra alone. Graphical abstract ᅟ.
由于离子淌度质谱在很大程度上具有不同于正交方法的气相分离特性，因此在结构糖生物学中具有相当大的应用潜力。在这里，我们评估了飞行波离子淌度与负离子碰撞诱导解离相结合在提供包含多个形成 Lewis 和 Lewis 表位的岩藻糖残基的 N-连接聚糖的结构信息方面的能力。这些表位参与细胞-细胞识别等过程，并且作为癌症生物标志物很重要。通过负离子 CID 从完整的 N-聚糖中可以获得的特定信息包括聚糖的一般拓扑结构，例如是否存在双分支 GlcNAc 残基和三天线聚糖的分支模式。每个天线特有的糖的位置信息也很容易获得。在离子淌度之前产生的一些等质量碎片离子随后可以被分离，并且在某些情况下，提供了来自 CID 光谱单独缺失的额外有价值的结构信息。
