Zhu Fa-Ming, Jiang Kan, Lü Qin-Feng, Zhang Wei, Zhang Hai-Qin, Fu Qi-Hua, Yan Li-Xing
Blood Center of Zhejiang Province, Hangzhou 310006, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2005 Dec;13(6):1109-12.
To analyze killer immunoglobulin (Ig)-like receptor (KIR) gene content and allelic polymorphism in Zhejiang Han population, samples were genotyped by polymerase chain reaction sequence-specific primers (PCR-SSP). The results demonstrated that all 17 KIR genes could be observed in the population. All individuals contained 2DL4, 3DL2 and 3DL3 genes. The frequencies of these genes was 1.00. 2DL1, 2DL3, 2DP1, 3DP1003, 3DL1, 2DS4001/002 loci were more common, their frequencies were 0.902, 0.902, 0.902, 0.902, 0.7598, 0.5615 respectively, while the frequencies of 2DL2, 2DL5A, 2DL5B, 2DS1, 2DS2, 2DS3, 2DS4003, 2DS5, 3DS1 and 3DP1001/002 were relatively lower. The A KIR haplotype was the most prevalent (74.7%) in Zhejiang Han population and there were 12 different KIR haplotypes in total, the most common was 2 (53.0%). Twenty six different genotypes have been found in the population, AJ (2, 2) and AF (1, 2) showed higher frequencies, followed by AH (2, 5), NN2 (2, 6), AI (1, 5) and AG (1, 1). Fifteen of these genotypes have not been found in Caucasians so far and four new KIR profiles could not be assigned to the haplotypes according to standard assign method. In conclusion, there are distinctive frequencies of KIR gene content, haplotype as well as genotype in Zhejiang Han population.
为分析浙江汉族人群中杀伤细胞免疫球蛋白样受体（KIR）基因组成及等位基因多态性，采用聚合酶链反应序列特异性引物（PCR-SSP）对样本进行基因分型。结果显示，该人群中可观察到全部17个KIR基因。所有个体均含有2DL4、3DL2和3DL3基因，这些基因的频率均为1.00。2DL1、2DL3、2DP1、3DP1003、3DL1、2DS4001/002位点较为常见，其频率分别为0.902、0.902、0.902、0.902、0.7598、0.5615，而2DL2、2DL5A、2DL5B、2DS1、2DS2、2DS3、2DS4003、2DS5、3DS1和3DP1001/002的频率相对较低。A KIR单倍型在浙江汉族人群中最为常见（74.7%），共有12种不同的KIR单倍型，最常见的是2（53.0%）。该人群中发现了26种不同的基因型，AJ（2, 2）和AF（1, 2）频率较高，其次是AH（2, 5）、NN2（2, 6）、AI（1, 5）和AG（1, 1）。其中15种基因型迄今在高加索人群中未被发现，且有4种新的KIR图谱根据标准分型方法无法归类到单倍型。总之，浙江汉族人群中KIR基因组成、单倍型及基因型频率具有独特性。
