Freccero Carolin, Wollmer Per, Sundkvist Göran, Svensson Henry
Department of Plastic and Reconstructive Surgery, Malmö University Hospital, University of Lund, SE-20502 Malmö, Sweden.
Microvasc Res. 2006 Jan;71(1):64-7. doi: 10.1016/j.mvr.2005.11.002. Epub 2006 Jan 5.
The aim of this study was to establish the degree to which a standardized test based on laser Doppler blood flow measurement is dependent on the particular equipment set-up being used. For this purpose, we examined finger skin blood flow with laser Doppler instruments in 20 healthy subjects. In laser Doppler perfusion monitoring (LDPM), we used a custom-made probe with two detecting fibers placed 0.25 and 1.2 mm from the illuminating fiber, respectively, and two laser Doppler perfusion imagers (LDPI) with a wavelength of 632.8 nm and 780 nm, respectively. Warming of the hand was achieved with a Peltier element, and reflex vasoconstriction was induced by immersing the other hand for 3 min into a water bath kept at 15 degrees C. As a measure for the change in skin blood flow, a vasoconstriction index (VAC: cooling/before cooling) was calculated and used for the comparison of the different devices. VAC values gathered around 0.6 for all devices. However, LDPI with a wavelength of 632.8 nm showed a slightly higher VAC index, and the difference was significant. We conclude that using a standardized test is the most appropriate for monitoring changes in blood flow rather than recording and comparing discrete values in intermittent recordings. Although a difference was noted when comparing the devices, different fiber separations and wavelengths seem then to be of little consequence.
本研究的目的是确定基于激光多普勒血流测量的标准化测试在多大程度上依赖于所使用的特定设备设置。为此，我们使用激光多普勒仪器在20名健康受试者中检测了手指皮肤血流。在激光多普勒灌注监测（LDPM）中，我们使用了一个定制探头，其两根检测光纤分别与照明光纤相距0.25毫米和1.2毫米，以及两台激光多普勒灌注成像仪（LDPI），其波长分别为632.8纳米和780纳米。通过一个珀耳帖元件对手部进行加热，并通过将另一只手浸入保持在15摄氏度的水浴中3分钟来诱发反射性血管收缩。作为皮肤血流变化的一种测量方法，计算了血管收缩指数（VAC：冷却后/冷却前），并用于比较不同的设备。所有设备的VAC值都集中在0.6左右。然而，波长为632.8纳米的LDPI显示出略高的VAC指数，且差异显著。我们得出结论，使用标准化测试最适合监测血流变化，而不是在间歇性记录中记录和比较离散值。尽管在比较设备时发现了差异，但不同的光纤间距和波长似乎影响不大。
