Huang Gang-Liang, Zhang Da-Wei, Zhao Hong-Juan, Zhang Hou-Cheng, Wang Peng-George
School of Life Science, Shandong University, Jinan City 250100, China.
Bioorg Med Chem Lett. 2006 Apr 1;16(7):2042-3. doi: 10.1016/j.bmcl.2005.12.055. Epub 2006 Jan 5.
Cell density cultivation of recombinant Escherichia coli strains harboring the nodC gene (encoding chitooligosaccharide synthase) from Azorhizobium caulinodans has been previously described as a practical method for the preparation of gram-scale quantities of penta-N-acetyl-chitopentaose. We have now extended this method to the production of allylated derivative of penta-N-acetyl-chitopentaose by using allyl 2-acetamido-2-deoxy-beta-d-glucopyranoside (2) as the initial acceptor for the synthesis of target pentaoside in vivo.
先前已描述了携带来自茎瘤固氮根瘤菌的nodC基因（编码壳寡糖合酶）的重组大肠杆菌菌株的细胞密度培养，这是一种制备克级量的五-N-乙酰基壳五糖的实用方法。我们现在已将此方法扩展至通过使用烯丙基2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖苷（2）作为体内合成目标五糖的初始受体来生产五-N-乙酰基壳五糖的烯丙基化衍生物。
