Huang Gang-Liang, Mei Xin-Ya
State Key Laboratory of Microbial Technology (SKLMT), Shandong University, Jinan, Shandong 250100, People's Republic of China.
J Enzyme Inhib Med Chem. 2007 Apr;22(2):247-9. doi: 10.1080/14756360601073740.
The penta-N-acetyl-chitopentaose 2 has been prepared by using recombinant E. coli strains harboring the nodC gene (encoding chitooligosaccharide synthase) from Azorhizobium caulinodans. Then, the deacetylase NodB removed the N-acetyl moiety from the nonreducing terminus of 2 to give tetra-N-acetyl-chitopentaose 3. N-Acylation of 3 with stearyl chloride was performed in DMF containing water and provided the corresponding lipo-chitopentaose nodulation factor 4. A binding chitinase assay indicated that 4 was much more stable than 3.
通过使用携带来自茎瘤固氮根瘤菌的nodC基因（编码壳寡糖合酶）的重组大肠杆菌菌株制备了五-N-乙酰-壳五糖2。然后，脱乙酰酶NodB从2的非还原末端去除N-乙酰部分，得到四-N-乙酰-壳五糖3。在含有水的N,N-二甲基甲酰胺中用硬脂酰氯对3进行N-酰化，得到相应的脂壳五糖结瘤因子4。结合几丁质酶测定表明4比3稳定得多。
