Valiunas Deividas, Staniulis Juozas, Davis Robert E
Laboratory of Phytoviruses, Institute of Botany, Zaliuju ezeru 49, Vilnius LT-08406, Lithuania.
Int J Syst Evol Microbiol. 2006 Jan;56(Pt 1):277-81. doi: 10.1099/ijs.0.63935-0.
Symptoms of general stunting and yellowing of leaves were observed in diseased cultivated strawberry (Fragaria x ananassa Duchesne) in Lithuania. Analysis of 16S rRNA gene sequences amplified by PCR indicated that the symptoms were associated with infection by a phytoplasma, designated strawberry yellows (StrawY) phytoplasma. Phylogenetic analysis of 16S rRNA gene sequences indicated that StrawY phytoplasma, 'Candidatus Phytoplasma australiense', 'Candidatus Phytoplasma asteris', stolbur phytoplasma and 'Candidatus Phytoplasma japonicum' shared a common ancestor, but were mutually distinct. Nucleotide sequence alignments of a 1.3 kb 16S rRNA gene sequence fragment revealed that StrawY phytoplasma shared 97.4 % or less similarity with previously described 'Candidatus Phytoplasma' species. These results, in addition to natural host and geographical occurrence, support the recognition of StrawY phytoplasma as a representative of a novel taxon, 'Candidatus Phytoplasma fragariae'.
在立陶宛，患病的栽培草莓（凤梨草莓，Fragaria x ananassa Duchesne）出现了植株普遍发育不良和叶片发黄的症状。通过聚合酶链反应（PCR）扩增16S rRNA基因序列进行分析表明，这些症状与一种植原体感染有关，该植原体被命名为草莓黄化（StrawY）植原体。对16S rRNA基因序列进行系统发育分析表明，草莓黄化植原体、“澳大利亚植原体”（Candidatus Phytoplasma australiense）、“紫菀植原体”（Candidatus Phytoplasma asteris）、 stolbur植原体和“日本植原体”（Candidatus Phytoplasma japonicum）有一个共同的祖先，但彼此不同。对一个1.3 kb的16S rRNA基因序列片段进行核苷酸序列比对发现，草莓黄化植原体与先前描述的“植原体属”（Candidatus Phytoplasma）物种的相似性为97.4%或更低。这些结果，连同其自然寄主和地理分布情况，支持将草莓黄化植原体认定为一个新分类单元“草莓植原体”（Candidatus Phytoplasma fragariae）的代表。
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