柳树肿病防治的新视角：水杨酸对疾病控制的作用及转录组和蛋白质组变化的研究。

Novel Insight into the Prevention and Therapeutic Treatment of Witches' Broom: A Study on the Effect of Salicylic Acid on Disease Control and the Changes in the Transcriptome and Proteome.

机构信息

College of Forestry, Henan Agricultural University, Zhengzhou 450002, China.

Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China.

出版信息

Int J Mol Sci. 2024 Sep 30;25(19):10553. doi: 10.3390/ijms251910553.

DOI:10.3390/ijms251910553

PMID:39408878

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11476412/

Abstract

species not only have significant economic benefits but also show great potential in ecological conservation. However, they are highly susceptible to phytoplasma infections, causing witches' broom (PaWB), which severely restricts the development of the industry. Salicylic acid (SA) plays a crucial role in plant disease resistance. However, there have been no reports on the effect of SA on PaWB. Due to the properties of SA, it may have potential in controlling PaWB. Based on the above speculation, the prevention and therapeutic effect of SA on PaWB and its effect on the PaWB-infected transcriptome and proteome were studied in this work. The results indicated that 0.1 mmol/L was the optimal SA concentration for inhibiting the germination of axillary buds. In terms of resistance physiological indicators, SA treatment significantly affected both infected (PTI) seedlings and infected (PFI) seedlings, where the activities of peroxidase (POD) and superoxide dismutase (SOD) were enhanced. Malondialdehyde (MDA), O, and HO, however, were significantly reduced. Specifically, after SA treatment, SOD activity increased by 28% in PFI and 25% in PTI, and POD activity significantly increased by 61% in PFI and 58% in PTI. Moreover, the MDA content decreased by 30% in PFI and 23% in PTI, the HO content decreased by 26% in PFI and 19% in PTI, and the O content decreased by 21% in PFI and 19% in PTI. Transcriptomic analysis showed that there were significant upregulations of MYB, NAC, and bHLH and other transcription factors after SA treatment. Moreover, genes involved in PaWB-related defense responses such as RAX2 also showed significant differences. Furthermore, proteomic analysis indicated that after SA treatment, proteins involved in signal transduction, protein synthesis modification, and disease defense were differentially expressed. This work provides a research foundation for the prevention and treatment of PaWB and offers references for exploring anti-PaWB methods.

摘要

物种不仅具有重要的经济效益，而且在生态保护方面也具有巨大的潜力。然而，它们极易受到植原体的感染，导致丛枝病（PaWB），这严重限制了该产业的发展。水杨酸（SA）在植物抗病性中起着至关重要的作用。然而，目前还没有关于 SA 对 PaWB 影响的报道。由于 SA 的特性，它可能在控制 PaWB 方面具有潜力。基于上述推测，本研究探讨了 SA 对 PaWB 的预防和治疗作用及其对 PaWB 感染的转录组和蛋白质组的影响。结果表明，0.1mmol/L 是抑制侧芽萌发的最佳 SA 浓度。就抗性生理指标而言，SA 处理显著影响感染（PTI）和感染（PFI）的幼苗，其中过氧化物酶（POD）和超氧化物歧化酶（SOD）的活性增强。丙二醛（MDA）、O 和 HO 则显著减少。具体而言，SA 处理后，PFI 中 SOD 活性增加 28%，PTI 中增加 25%，PFI 中 POD 活性显著增加 61%，PTI 中增加 58%。此外，PFI 中 MDA 含量减少 30%，PTI 中减少 23%，PFI 中 HO 含量减少 26%，PTI 中减少 19%，PFI 中 O 含量减少 21%，PTI 中减少 19%。转录组分析表明，SA 处理后，MYB、NAC 和 bHLH 等转录因子的表达显著上调。此外，与 PaWB 相关的防御反应相关基因如 RAX2 也表现出显著差异。此外，蛋白质组分析表明，SA 处理后，信号转导、蛋白质合成修饰和疾病防御相关的蛋白质表达水平发生了差异。这项工作为 PaWB 的防治提供了研究基础，为探索抗 PaWB 方法提供了参考。