Correia Alexandra, Sampaio Paula, James Steve, Pais Célia
Centro de Biologia da Universidade do Minho (CBUM), Departamento de Biologia, Campus de Gualtar, 4710-057 Braga, Portugal.
Int J Syst Evol Microbiol. 2006 Jan;56(Pt 1):313-7. doi: 10.1099/ijs.0.64076-0.
Two yeast strains, 153MT and NCYC 3133, isolated from clinical sources in separate hospitals were found to be almost identical in the sequences of the D1/D2 domain of large-subunit rDNA, the PCR fingerprinting profiles and physiological characteristics. The isolates are phenotypically similar, although not identical, to Candida glabrata and Kluyveromyces delphensis (recently renamed Nakaseomyces delphensis). Sequence analysis of the 26S rDNA D1/D2 gene variable region revealed that the two clinical isolates were closely related phylogenetically to C. glabrata and K. delphensis, but differed sufficiently to justify their assignment as representatives of a separate species. The name Candida bracarensis sp. nov. is proposed for the novel species with the type strain 153MT (= CBS 10154T = NCYC D3853T = CECT 12000T).
从不同医院的临床样本中分离出的两株酵母菌株153MT和NCYC 3133,在大亚基核糖体DNA的D1/D2结构域序列、PCR指纹图谱和生理特征方面几乎完全相同。这些分离株在表型上与光滑念珠菌和德尔芬克鲁维酵母(最近重新命名为纳卡酵母)相似,但并不完全相同。对26S rDNA D1/D2基因可变区的序列分析表明,这两株临床分离株在系统发育上与光滑念珠菌和德尔芬克鲁维酵母密切相关,但差异足以将它们归为一个单独物种的代表。提议将这个新物种命名为布拉加念珠菌(Candida bracarensis sp. nov.),模式菌株为153MT(= CBS 10154T = NCYC D3853T = CECT 12000T)。