Spalteholz Holger, Wenske Kathrin, Arnhold Jürgen
Institute of Medical Physics and Biophysics, Medical Faculty, University of Leipzig, Leipzig, Germany.
Biofactors. 2005;24(1-4):67-76. doi: 10.1002/biof.5520240108.
The formation of chlorohydrins, bromohydrins, and iodohydrins from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) by the myeloperoxidase-hydrogen peroxide-halide system was evaluated by means of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry. This approach allows to detect different kinds of the halogenation reaction even in one mass spectrum. Using a mixture of Cl-, Br-, I-, and SCN- at physiological concentrations, a bromination of POPC dominates by the MPO-hydrogen peroxide-halide system. Hypothiocyanite does apparently not react with the double bond of POPC, but increasing amounts of SCN- cause a decrease of the bromohydrin peaks. An interconversion between different hypohalous acids produced by the myeloperoxidase-hydrogen peroxide-halide system determines the pattern of halogenohydrins in POPC. Especially, hypochlorous acid is able to oxidise Br- to hypobromous acid.
通过基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法评估了髓过氧化物酶-过氧化氢-卤化物系统由1-棕榈酰-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）形成氯醇、溴醇和碘醇的情况。这种方法即使在一个质谱图中也能检测到不同类型的卤化反应。使用生理浓度的Cl-、Br-、I-和SCN-混合物时，MPO-过氧化氢-卤化物系统以POPC的溴化反应为主。次硫氰酸盐显然不与POPC的双键反应，但SCN-量的增加会导致溴醇峰减少。髓过氧化物酶-过氧化氢-卤化物系统产生的不同次卤酸之间的相互转化决定了POPC中卤醇的模式。特别是，次氯酸能够将Br-氧化为次溴酸。
