结节性硬化症复合物22蛋白在星形胶质细胞瘤中的表达模式。
Patterns of expression of TSC-22 protein in astrocytic gliomas.
作者信息
Shostak K O, Dmitrenko V V, Vudmaska M I, Naidenov V G, Beletskii A V, Malisheva T A, Semenova V M, Zozulya Yu P, Demotes-Mainard J, Kavsan V M
机构信息
Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, Ukraine.
出版信息
Exp Oncol. 2005 Dec;27(4):314-8.
AIM
To evaluate expression patterns of protein product of putative tumor suppressor gene TSC-22 in human astrocytic tumors by immunohistochemical approach.
METHODS
Plasmid pET-23d-TSC22 was constructed for the expression of human TSC-22 protein in bacterial system, and polyclonal rabbit antibodies against recombinant TSC-22 were produced. Immunohistochemical analysis of TSC-22 and GFAP expression with the use of anti-human-TSC-22- and anti-human-GFAP-antibodies was performed on histological slides of astrocytic tumors.
RESULTS
Immunohistochemical analysis has shown that the number of cells expressing TSC-22 was significantly lower in glioblastoma tissues than that in diffuse astrocytoma. Double immunohistochemical staining of astrocytic tumors using anti-human-TSC-2- and anti-human-GFAP-antibodies showed that both TSC-22 and GFAP expression is co-localized in astrocytes.
CONCLUSION
TSC-22 protein is expressed in astrocytes, but not in macrophage/microglial cells. In more aggressive forms of astrocytic tumors decreased expression of TSC-22 mRNA correlates with its lowered expression on protein level.
目的
采用免疫组化方法评估假定的肿瘤抑制基因TSC - 22的蛋白质产物在人星形细胞瘤中的表达模式。
方法
构建质粒pET - 23d - TSC22以在细菌系统中表达人TSC - 22蛋白，并制备针对重组TSC - 22的兔多克隆抗体。使用抗人TSC - 22抗体和抗人GFAP抗体对星形细胞瘤组织切片进行TSC - 22和GFAP表达的免疫组化分析。
结果
免疫组化分析表明，胶质母细胞瘤组织中表达TSC - 22的细胞数量明显低于弥漫性星形细胞瘤。使用抗人TSC - 2抗体和抗人GFAP抗体对星形细胞瘤进行双重免疫组化染色显示，TSC - 22和GFAP的表达均共定位于星形胶质细胞中。
结论
TSC - 22蛋白在星形胶质细胞中表达，但在巨噬细胞/小胶质细胞中不表达。在侵袭性更强的星形细胞瘤中，TSC - 22 mRNA表达降低与其蛋白水平表达降低相关。
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