Park Shin Ae, Lee Hyang Woo, Hong Myung Hee, Choi Young Wook, Choe Yon Ho, Ahn Bo Young, Cho Yang Je, Kim Dong Su, Lee Na Gyong
Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, Kwangjin-gu, Seoul, Korea.
Proteomics. 2006 Feb;6(4):1319-28. doi: 10.1002/pmic.200500293.
Helicobacter pylori is known to cause chronic gastritis, peptic ulcer, and gastric cancer, and has also been linked to iron deficiency anemia (IDA). To determine whether H. pylori clinical isolates correlate with the prevalence of H. pylori-associated IDA, we compared the proteomic profiles of H. pylori strains isolated from antral biopsy specimens of H. pylori-positive patients with or without IDA. Fifteen strains, including eight non-IDA and seven IDA strains, were cultured under iron-rich and iron-depleted conditions and then analyzed for protein expression profiles by 2-DE. The distances between two H. pylori strains were determined on the basis of similarities between their expression patterns of 189 protein spots, and a phylogenetic tree was constructed. The results revealed that the IDA strains formed a cluster separate from that of six non-IDA strains, with two non-IDA strains between the clusters. H. pylori strain 26695 was located in the non-IDA cluster. Protein spots displaying similar expression patterns were clustered, and 18 spots predominantly expressed in IDA strains were identified by MALDI-TOF analysis. These data indicate that the non-IDA and IDA strains can be distinguished by their protein expression profiles, suggesting that the polymorphism of H. pylori strains may be one of the factors determining the occurrence of H. pylori-associated IDA.
已知幽门螺杆菌可导致慢性胃炎、消化性溃疡和胃癌，还与缺铁性贫血（IDA）有关。为了确定幽门螺杆菌临床分离株是否与幽门螺杆菌相关IDA的患病率相关，我们比较了从有或无IDA的幽门螺杆菌阳性患者胃窦活检标本中分离出的幽门螺杆菌菌株的蛋白质组学图谱。15株菌株，包括8株非IDA菌株和7株IDA菌株，在富铁和缺铁条件下培养，然后通过二维电泳分析蛋白质表达谱。根据189个蛋白点的表达模式之间的相似性确定两株幽门螺杆菌之间的距离，并构建系统发育树。结果显示，IDA菌株形成了一个与6株非IDA菌株分开的簇，两簇之间有2株非IDA菌株。幽门螺杆菌菌株26695位于非IDA簇中。显示相似表达模式的蛋白点聚类，通过基质辅助激光解吸电离飞行时间分析鉴定出18个在IDA菌株中主要表达的蛋白点。这些数据表明，非IDA菌株和IDA菌株可通过其蛋白质表达谱进行区分，提示幽门螺杆菌菌株的多态性可能是决定幽门螺杆菌相关IDA发生的因素之一。
