You Yun-hui, Fang Xue-gong, Liu Ping, Liu Hong-bo, Tian Xue-fei, Yan Xue-mei
Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 May;33(5):384-90.
To establish 2-dimensional electrophoresis (2-DE) maps of Helicobac-ter pylori in human gastritis, and gastric cancer, to identify the differentially expressed proteins,and to discuss the role of bacterial factor in pathogenesis.
The total proteins of Helicobacter pylori in human gastritis and gastric cancer were separated by immobilized pH gradient-based 2-DE. The differentially expressed proteins were screened by PDQuest analysis software and identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flight mass spectrometry, and searched on database.
A well-resolved and reproducible 2-DE pattern of Helicobacter pylori was obtained from patients with human gastritis and gastric cancer. Fourteen differentially expressed proteins were identified, including proteins related to anti-oxidation,molecular chape-rones and detoxification, enzymes related to metabolism,proteins related to cytoarchitecture,and proteins related to signal conduction.
A well-resolved and reproducible 2-DE pattern of Helicobacter pylori in human gastritis and gastric cancer is established and differentially expressed proteins from these 2 diseases are identified. The differentiation of protein expression may play an important role in the pathogenesis of gastric cancer.
建立人胃炎及胃癌中幽门螺杆菌的二维电泳（2-DE）图谱，鉴定差异表达蛋白，并探讨细菌因素在发病机制中的作用。
采用基于固定化pH梯度的二维电泳分离人胃炎及胃癌中幽门螺杆菌的总蛋白。通过PDQuest分析软件筛选差异表达蛋白，并基于基质辅助激光解吸/电离飞行时间质谱的肽质量指纹图谱进行鉴定，同时在数据库中进行搜索。
从人胃炎及胃癌患者中获得了分辨率良好且可重复的幽门螺杆菌二维电泳图谱。鉴定出14种差异表达蛋白，包括与抗氧化、分子伴侣和解毒相关的蛋白、与代谢相关的酶、与细胞结构相关的蛋白以及与信号传导相关的蛋白。
建立了人胃炎及胃癌中分辨率良好且可重复的幽门螺杆菌二维电泳图谱，并鉴定了这两种疾病中的差异表达蛋白。蛋白表达差异可能在胃癌发病机制中起重要作用。
