2003年在哥伦比亚分离的禽传染性支气管炎病毒株的分子特征

Molecular characterization of avian infectious bronchitis virus strains isolated in Colombia during 2003.

作者信息

Alvarado I R, Villegas P, Mossos N, Jackwood M W

机构信息

Department of Avian Medicine, University of Georgia, Athens, GA 30602, USA.

出版信息

Avian Dis. 2005 Dec;49(4):494-9. doi: 10.1637/7202-050304R.1.

DOI:10.1637/7202-050304R.1

PMID:16404989

Abstract

Sixteen infectious bronchitis virus (IBV) isolates were recovered from broilers and layers from five geographic poultry regions in Colombia. The viruses were isolated from tracheas, lungs, and cecal tonsils of birds, previously vaccinated with the Massachusetts strain, that were showing respiratory signs. Further analysis of the IBV isolates was achieved by phylogenetic analysis comparing their deduced amino acid sequences in the hypervariable region 1 of the S1 gene with reference strains. Four unique genotype clusters containing isolates with indigenous genotypes were observed. One isolate was found to be the Connecticut genotype and three isolates were found to be the Massachusetts genotype.

摘要

从哥伦比亚五个地理家禽区域的肉鸡和蛋鸡中分离出16株传染性支气管炎病毒（IBV）毒株。这些病毒是从先前接种过马萨诸塞毒株但出现呼吸道症状的禽类的气管、肺和盲肠扁桃体中分离出来的。通过系统发育分析，将IBV毒株S1基因高变区1的推导氨基酸序列与参考毒株进行比较，对这些分离株进行了进一步分析。观察到四个独特的基因型簇，其中包含具有本土基因型的分离株。发现一株为康涅狄格基因型，三株为马萨诸塞基因型。