Chilingaryan Amaiak, Chilingaryan Amayak M, Martin Gary G
Department of Biology, Occidental College, Los Angeles, CA 90041, USA.
Brain Res. 2006 Jan 27;1070(1):131-8. doi: 10.1016/j.brainres.2005.11.059. Epub 2006 Jan 10.
A procedure is described which allows for the selective and non-injectional staining of the three-dimensional microvasculatory bed (MVB) in thick sections (60-140 microm) of formalin-fixed brain tissue of white rats Rattus norvegicus. This histochemical method detects ATPase activity and takes place between pH 10.5 and 11.2. Calcium ion is used to capture inorganic phosphate, calcium phosphate is converted to lead phosphate, and subsequently converted to black or dark brown lead sulfide. All vessels are revealed due to a precipitate on the endothelium and smooth muscle cells of arterioles. In some vessels, red blood cells also stain. The background is transparent with no staining of neurons, nerve fibers, glial cells, or nuclei. This allows for clear identification of arterioles, venules, and capillaries, which is difficult using other methods. New observations are described including the presence of webs connecting branching parts of arterioles and constrictions along vessels. This procedure should be useful in investigations of the MVB in rat brain.
本文描述了一种方法，可对福尔马林固定的大白鼠（褐家鼠）脑组织厚切片（60 - 140微米）中的三维微血管床（MVB）进行选择性且非注射性染色。这种组织化学方法检测ATP酶活性，在pH 10.5至11.2之间进行。钙离子用于捕获无机磷酸盐，磷酸钙转化为磷酸铅，随后转化为黑色或深棕色硫化铅。由于小动脉内皮和平滑肌细胞上有沉淀，所有血管均被显示出来。在一些血管中，红细胞也会染色。背景透明，神经元、神经纤维、胶质细胞或细胞核均无染色。这使得小动脉、小静脉和毛细血管能够清晰识别，而这用其他方法很难做到。文中描述了一些新的观察结果，包括存在连接小动脉分支部分的网以及沿血管的狭窄处。该方法在大鼠脑MVB的研究中应会有用。
