Gudlavalleti Seshu K, Szymanski Christine M, Jarrell Harold C, Stephens David S
Laboratory of Microbial Pathogenesis, VA Medical Center and Division of Infectious Diseases, Atlanta, GA, USA.
Carbohydr Res. 2006 Mar 20;341(4):557-62. doi: 10.1016/j.carres.2005.11.036. Epub 2006 Jan 9.
High resolution-magic angle spinning (HRMAS) NMR spectroscopy was applied to serogroup A Neisseria meningitidis (NMA) to determine precise structures of capsular polysaccharide (CPS) expressed on the meningococcal surface. Both the O-acetylated (OAc) NMA parent and a mynC::aphA3 OAc- mutant demonstrated characteristic CPS-derived NMR signals indicating cell-surface expression of CPS, but only the parent expressed O-3 and O-4 acetylation signals. A capsule-defective strain showed no NMR signals for CPS. The (1)H NMR HRMAS spectral patterns correlated with the purified CPS (1)H NMR profiles. HRMAS NMR can distinguish detailed complex carbohydrate structures expressed on bacteria. NMA express both O-3 and O-4 acetylated polymers but not in equimolar ratio amounts in vivo.
高分辨率魔角旋转（HRMAS）核磁共振波谱法被应用于A群脑膜炎奈瑟菌（NMA），以确定脑膜炎球菌表面表达的荚膜多糖（CPS）的精确结构。O-乙酰化（OAc）NMA亲本菌株和mynC::aphA3 OAc-突变体均显示出源自CPS的特征性核磁共振信号，表明CPS在细胞表面表达，但只有亲本菌株表达O-3和O-4乙酰化信号。一个荚膜缺陷菌株未显示出CPS的核磁共振信号。（1）H NMR HRMAS光谱模式与纯化的CPS（1）H NMR谱相关。HRMAS NMR可以区分细菌表面表达的详细复杂碳水化合物结构。NMA表达O-3和O-4乙酰化聚合物，但在体内并非等摩尔量。
