Kondo E, Kanai K
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Jpn J Med Sci Biol. 1992 Feb;45(1):19-34. doi: 10.7883/yoken1952.45.19.
In a whole cell assay system with p-nitrophenyl phosphate as substrate, strains of Mycobacterium tuberculosis and M. bovis were identical in the pH-activity pattern of acid phosphatase. It was a one-peak curve with a pH optimum at 6.2 and sharp symmetrical slopes. The enzymatic activity did not reflect the virulence. When the cells were subjected to mechanical fractionation, the major part of the enzymatic activity was found in a particulate fraction and a minor portion in supernatant and cell walls, suggesting the location of the enzyme in the membrane. Exposure of the cells to free long-chain fatty acids, especially unsaturated ones, reduced the enzymatic activity in a dose-response manner with concomitant decrease in the viability. However, no causal relationship between these two effects was suggested from the collateral experiments.
在以对硝基苯磷酸为底物的全细胞检测系统中，结核分枝杆菌菌株和牛分枝杆菌在酸性磷酸酶的pH活性模式上是相同的。它是一条单峰曲线，最适pH值为6.2，斜率尖锐且对称。酶活性并未反映出毒力。当细胞进行机械分级分离时，大部分酶活性存在于颗粒部分，少量存在于上清液和细胞壁中，这表明该酶位于膜中。将细胞暴露于游离长链脂肪酸，尤其是不饱和脂肪酸，会以剂量反应方式降低酶活性，同时细胞活力也会下降。然而，这些平行实验并未表明这两种效应之间存在因果关系。
