Kondo E, Dejsirilert S, Wejprasit N, Chiewsilp D, Kanai K
Department of Medical Sciences, Ministry of Public Health, Soi Bamrasnaradura Hospital, Nonthaburi, Thailand.
Jpn J Med Sci Biol. 1991 Apr;44(2):51-62. doi: 10.7883/yoken1952.44.51.
In a whole cell assay system with p-nitrophenyl phosphate as substrate, strains of Pseudomonas pseudomallei showed a two-peak pattern in pH activity curve of acid phosphatase, suggesting the presence of two enzyme components different in pH optimum (4.2 and 5.2). The component of 5.2 pH optimum was detected in the outer membrane fraction and the activity was resistant to heating at 70 C for 30 min. The other component of 4.2 pH optimum was heat-labile. No substantial difference was observed in the enzymatic activity between R and S type colonies.
在以对硝基苯磷酸为底物的全细胞分析系统中，类鼻疽假单胞菌菌株在酸性磷酸酶的pH活性曲线中呈现双峰模式，表明存在两种最适pH不同（4.2和5.2）的酶组分。最适pH为5.2的组分在外膜部分被检测到，其活性在70℃加热30分钟后仍保持稳定。另一种最适pH为4.2的组分对热不稳定。R型和S型菌落之间的酶活性未观察到显著差异。
