Houng Huo-Shu H, Clavio Sarah, Graham Katherine, Kuschner Robert, Sun Wellington, Russell Kevin L, Binn Leonard N
Department of Virus Diseases, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
J Clin Virol. 2006 Apr;35(4):381-7. doi: 10.1016/j.jcv.2005.11.008. Epub 2006 Jan 6.
Ad4 is the principal etiological agent of acute respiratory disease (ARD) in the US military. Discovery of the novel 208bp inverted terminal repeated (ITR) sequence from a recent Ad4 Jax78 field isolate was totally distinct from the analogous 116bp ITR of Ad4 prototype.
To investigate the origin and distribution of the novel Ad4 ITR sequence from ARD infections.
Direct sequencing of ligated Ad ITR termini.
The new Ad4 ITR was highly homologous with the ITRs of human Ad subgroup B. The left post-ITR region of Ad4 Jax78 was found to be highly homologous to the corresponding region of subgroup B Ads: 81% for Ad11 and 98% for Ad3 and Ad7. The right post-ITR region of Ad4 Jax78 contained a truncated classic ITR of the Ad4 prototype.
The Ad4 Jax78 ITR most likely evolved from Ad4 prototype by substituting the Ad4 prototype ITR with the subgroup B Ads ITR. The ITR-based PCR assays developed from this study can be used to distinguish the new Ad4 genotype from the classical Ad4 prototype. The new Ad4 genotype was first detected in 1976 from Georgia, USA, and is the main causative agent of ARD infections in US military population.
腺病毒4型（Ad4）是美国军队急性呼吸道疾病（ARD）的主要病原体。从最近分离的Ad4 Jax78野外毒株中发现的新型208bp反向末端重复序列（ITR）与Ad4原型的类似116bp ITR完全不同。
研究来自ARD感染的新型Ad4 ITR序列的起源和分布。
对连接的腺病毒ITR末端进行直接测序。
新的Ad4 ITR与人腺病毒B亚组的ITR高度同源。发现Ad4 Jax78的左ITR后区域与B亚组腺病毒的相应区域高度同源：与Ad11的同源性为81%，与Ad3和Ad7的同源性为98%。Ad4 Jax78的右ITR后区域包含Ad4原型的截短经典ITR。
Ad4 Jax78 ITR很可能是通过用B亚组腺病毒的ITR替代Ad4原型的ITR从Ad4原型进化而来。本研究开发的基于ITR的PCR检测方法可用于区分新的Ad4基因型与经典的Ad4原型。新的Ad4基因型于1976年首次在美国佐治亚州被检测到，是美国军队人群中ARD感染的主要病原体。
