N,N'-二乙基间甲苯酰胺（m-DET）：采用高效液相色谱法分析人尿液和血清中的一种驱虫剂
N,N'-diethyl-m-toluamide (m-DET): analysis of an insect repellent in human urine and serum by high-performance liquid chromatography.
作者信息
Smallwood A W, DeBord K E, Lowry L K
机构信息
Food and Drug Administration, National Forensic Chemistry Center, Cincinnati, Ohio 45202.
出版信息
J Anal Toxicol. 1992 Jan-Feb;16(1):10-3. doi: 10.1093/jat/16.1.10.
A procedure for monitoring m-DET in human urine and serum is described. m-DET is removed from the urine specimen by partitioning into diethyl ether, but solid-phase extraction is used to remove it from human serum. The urine and serum m-DET values are determined by HPLC with a UV detector. The limit of detection was 0.09 micrograms/mL in urine and 0.09 micrograms/g for serum. The percent of m-DET recovered from human urine spiked between 0.50 and 8.00 micrograms/mL was 90 +/- 5.4%. For human serum spiked between 0.25 and 10.00, the percent recovered was 96 +/- 5.9%. The pooled relative standard deviations (RSD) for spiked matrices were 0.06 for urine and 0.06 for serum.
本文描述了一种监测人体尿液和血清中m-DET的方法。通过用二乙醚萃取从尿液样本中去除m-DET，但从人体血清中去除m-DET则采用固相萃取法。尿液和血清中的m-DET值通过配备紫外检测器的高效液相色谱法测定。尿液中的检测限为0.09微克/毫升，血清中的检测限为0.09微克/克。在添加了0.50至8.00微克/毫升m-DET的人体尿液中，回收率为90±5.4%。在添加了0.25至10.00微克/毫升m-DET的人体血清中，回收率为96±5.9%。加标基质的合并相对标准偏差（RSD），尿液为0.06，血清为0.06。
Zotero 插件