Kraut E H, Husney R M, Sagone A L
Ohio State University, Department of Internal Medicine, Columbus 43210.
Leukemia. 1992 Aug;6(8):834-8.
Hairy cell leukemia is a malignant B-cell disorder characterized by splenomegaly and pancytopenia. The malignant cell is morphologically unique and characterized by fine cytoplasmic projections. Although studies of the cell have revealed important information about its proliferative capacity, cell surface, and membrane composition, less is known about the metabolic characteristics of the cell. We have previously investigated the oxidative metabolism of the hairy cell and have suggested that hairy cells might have a unique glucose metabolism compared to normal lymphocytes. This is indicated by a high rate of [6-14C]glucose oxidation in short-term culture consistent with an active Kreb's cycle and a high ratio of [6-14C]glucose oxidation to [1-14C] glucose oxidation. In this study, we evaluated an additional group of patients with hairy cell leukemia prior to or after treatment with the experimental drug 2'-deoxycoformycin (dCF). We found that in seven of eight patients the leukemic cells had a pattern similar to that previously described and that all of these seven patients had a significant response to therapy. The cells of the eighth patient had minimal Kreb's cycle activity, and at the time of study the patient was resistant to therapy with dCF. The metabolic activity of hairy cells may distinguish them from other lymphoid populations and may be a marker for sensitivity to dCF.
毛细胞白血病是一种恶性B细胞疾病，其特征为脾肿大和全血细胞减少。恶性细胞在形态上具有独特性，其特点是有细小的胞质突起。尽管对该细胞的研究已经揭示了有关其增殖能力、细胞表面和膜组成的重要信息，但对该细胞的代谢特征了解较少。我们之前研究了毛细胞的氧化代谢，并提出与正常淋巴细胞相比，毛细胞可能具有独特的葡萄糖代谢。这表现为短期培养中[6-14C]葡萄糖氧化率高，与活跃的三羧酸循环一致，且[6-14C]葡萄糖氧化与[1-14C]葡萄糖氧化的比率高。在本研究中，我们评估了另一组在接受实验性药物2'-脱氧助间型霉素（dCF）治疗之前或之后的毛细胞白血病患者。我们发现，8名患者中有7名患者的白血病细胞具有与先前描述相似的模式，并且这7名患者均对治疗有显著反应。第8名患者的细胞三羧酸循环活性最低，在研究时该患者对dCF治疗耐药。毛细胞的代谢活性可能使其与其他淋巴细胞群体区分开来，并且可能是对dCF敏感性的一个标志物。
